Your best choice for trouble-free IP-WB experiments |
| Immunoprecipitation (IP) and co-immunoprecipitation (co-IP) assays often necessitate the use of the same antibody, or antibodies from the same host, during both the IP and western blot (WB) procedures. Denatured immunoglobulin (Ig) in the IP sample can be bound by the secondary antibody used for the WB. Thus, depending on the molecular weight of the target protein, the desired band may be partially or completely obscured on the WB image by signal from denatured heavy (-50 kDa) and/or light (-25 kDa) Ig chains. |
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GeneTex's EasyBlot® secondary antibodies are specifically optimized to eliminate this problem. They bind only the native (non-denatured and non-reduced) primary antibody used for WB analysis and not the denatured IP antibody chains. In addition, the specially formulated EasyBlocker reagent will greatly reduce background signal caused by free Protein A or Protein G. |
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EasyBlot®secondary antibodies are indispensable for achieving the cleanest results for your IP-WB experiments. |
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EasyBlot®: The easiest way to get the best result! |
| Clear |
| Efficiently eliminates denatured IgG masking of your target protein's signal |
| Compatible |
| Suitable for WB following either IP or co-IP analysis using Protein A-, Protein G-, or Agarose-conjugated antibodies |
| Comprehensive |
| Broad range of EasyBlot® reagents for detecting various hosts: rabbit, mouse, rat, goat or sheep |
| Convenient: Simple and user-friendly! |
| Easy to switch from regular secondary antibodies to EasyBlot® secondary antibodies |
| Combine with EasyBlocker to further optimize your specific signal |
| Significantly reduce background generated by free Protein A/G with the EasyBlocker reagent. |
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