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同型对照

同型对照

Inclusion of an appropriate isotype control is essential for the proper interpretation of experimental results. Because isotype control antibodies have no relevant specificity, they enable the researcher to distinguish non-specific "background" binding from antigen-specific antibody binding. Non-specific binding of primary antibodies may occur due to either binding of antibody to Fc receptors or non-specific interactions with cellular proteins, lipids, or carbohydrates. Additionally, autofluorescence or endogenous enzyme activity in target cells may give a false positive that could be misinterpreted as antibody binding.

These factors vary by target cell type and the isotype of the primary antibody. Therefore, the primary antibody used should be matched to an isotype control of identical species and isotype (including both heavy and light chains).

 

人类免疫球蛋白

人类免疫球蛋白
兔免疫球蛋白

兔免疫球蛋白
小鼠免疫球蛋白

小鼠免疫球蛋白
 
大鼠免疫球蛋白

大鼠免疫球蛋白
其他同型对照

其他同型对照
山羊免疫球蛋白

山羊免疫球蛋白
 
绵羊免疫球蛋白

绵羊免疫球蛋白
仓鼠免疫球蛋白

仓鼠免疫球蛋白
   
Literature

单页 - Cited Antibodies 2024 Q2

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Literature

折页 - 热门产品

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Protocols

实验步骤 - 夹心法ELISA

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Protocols

实验步骤 - 间接ELISA

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