4E-BP1 (phospho Thr37/Thr46)抗体 [HL3276]

Untreated (–) and treated (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with 4E-BP1 (phospho Thr37/Thr46) antibody [HL3276] (GTX640930) diluted at 1:5000 and 4E-BP1 antibody (GTX109162) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Untreated (–) and treated (+) NIH-3T3 whole cell extract (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with 4E-BP1 (phospho Thr37/Thr46) antibody [HL3276] (GTX640930) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.