5-Hydroxymethylcytosine / 5-hmC抗体

GTX128454 5-Hydroxymethylcytosine/ 5-hmC antibody in MeDIP experiment.
Human genomic DNA (150 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Hydroxymethylcytosine/ 5-hmC antibody (GTX128454) at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

GTX128454 5-hydroxymethylcytosine antibody Dot blot analysis of anti-5-hmC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng as indicated) were spotted onto the positively charged Nylon membrane and blotted with 5-hmC antibody (GTX128454) at a dilution of 1:50000.
A: Unmethylated DNA
B: DNA containing 5-methylcytosine
C: DNA containing 5-hydroxymethylcytosine
D: DNA containing 5-formylcytosine
E: DNA containing 5-carboxylcytosine