5-Methylcytosine / 5-mC抗体

GTX128455 5-Methylcytosine/ 5-mC antibody in MeDIP experiment.
Human genomic DNA (150 ng) mixed with methylated cytosine standard kit (GTX400004) were subjected to MeDIP with 5-Methylcytosine/ 5-mC antibody (GTX128455) at dilution of 1:2000 and analyzed with semi-quantitative PCR. The immunoprecipitated DNA was plotted as % of input DNA.

GTX128455 5-methylcytosine antibody Dot blot analysis of anti-5-mC antibody with the synthetic DNA controls (GTX400004). DNA samples (0.5 to 50 ng) were spotted onto positively charged nylon membrane and blotted with 5-mC antibody (GTX128455) at a dilution of 1:4000.
A: Unmethylated DNA
B: DNA containing 5-methylcytosine
C: DNA containing 5-hydroxymethylcytosine
D: DNA containing 5-formylcytosine
E: DNA containing 5-carboxylcytosine