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53BP1抗体 [N1], N-term

KO/KD
Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595
Anti-53BP1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102595
Anti-53BP1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102595
Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595
Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595

目录号 GTX102595

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 53BP1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

214 kDa. ( Note )

阳性对照

293T , HeLa , Neuro 2A , C8D30 , NIH-3T3

产品属性

形式

Liquid

存储溶液

0.1M Tris, 0.1M Glycine, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human 53BP1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036128

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

tumor protein p53 binding protein 1 , 53BP1 , TDRD30 , p202 , p53BP1

细胞定位

Nucleus , Kinetochore

功能与背景

May have a role in checkpoint signaling during mitosis (By similarity). Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.

数据库

研究领域

產品图片

Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595

GTX102595 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

Anti-53BP1 antibody [N1], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102595

GTX102595 ICC/IF Image

53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: 53BP1 protein stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Anti-53BP1 antibody [N1], N-term used in IHC (Paraffin sections) (IHC-P). GTX102595

GTX102595 IHC-P Image

53BP1 antibody [N1], N-term detects 53BP1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
53BP1 stained by 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595

GTX102595 WB Image

53BP1 antibody [N1], N-term detects TP53BP1 protein by Western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
5 % SDS-PAGE
53BP1 antibody [N1], N-term (GTX102595) dilution: 1:500

Anti-53BP1 antibody [N1], N-term used in Western Blot (WB). GTX102595

GTX102595 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with 53BP1 antibody [N1], N-term (GTX102595) diluted at 1:500.

文献引用

Application Reference

Uyeda A et al. J Neurosci 2020; 40 (47) : 9012-9027 Suppression of DNA Double-Strand Break Formation by DNA Polymerase β in Active DNA Demethylation Is Required for Development of Hippocampal Pyramidal Neurons.
实验应用 : IHC-Fr
种属反应 : Mouse
Onishi K et al. J Neurosci 2017; (Epub) Genome Stability by DNA polymerase β in Neural Progenitors Contributes to Neuronal Differentiation in Cortical Development.
实验应用 : WB
种属反应 : Mouse
Chang HY et al. PLoS One 2013; 8 (1) : e54117 Middle infrared radiation induces G2/M cell cycle arrest in A549 lung cancer cells.
实验应用 : ICC/IF
种属反应 : Human
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Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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