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6X His tag抗体 [GT161]

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921
Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921
Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921
Anti-6X His tag antibody [GT161] used in ELISA (ELISA). GTX628921
Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

目录号 GTX628921

文献引用 1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT161

同种型

 IgG2a

实验应用

 WB, ELISA

种属反应

 Species independent
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ELISA 1:1000-1:10000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

His-tagged proteins

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to 6X His tag

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888083

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

6X His tag antibody , His antibody , 6x His antibody , 6x his tag antibody , His Tag antibody , 6xHis Tag antibody , 6 x His antibody

研究领域

產品图片

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

GTX628921 WB Image

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
A. Non-induced cell lysate from E. coli expressing His-tagged proteins
B. IPTG-induced cell lysate from E. coli expressing His-tagged proteins
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

GTX628921 WB Image

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
Lane A and B were IPTG-induced cell lysate from E. coli expressing different GST-tagged proteins.
Lane C to H were IPTG induced cell lysate from E. coli expressing different His-tagged protein.
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

GTX628921 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with 6X His tag antibody [GT161] (GTX628921) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-6X His tag antibody [GT161] used in ELISA (ELISA). GTX628921

GTX628921 ELISA Image

6X His tag antibody [GT161] detects His-tagged recombinant proteins by indirect ELISA analysis. Various dilutions of His-tagged recombinant proteins coated onto the 96-well plate were detected by using 0.5 μg/ml 6X His tag antibody [GT161] (GTX628914). Bound Abs were further detected by using HRP-conjugated goat anti-mouse IgG (GTX213111-01) with HRP substrates.

Anti-6X His tag antibody [GT161] used in Western Blot (WB). GTX628921

GTX628921 WB Image

6X His tag antibody [GT161] detects 6xHis Tag protein by western blot analysis.
A. 2 μg GST-recombinant protein
B. 2 μg His-recombinant protein
C. 1 μg His-recombinant protein
D. 0.5 μg His-recombinant protein
E. 0.1 μg His-recombinant protein
12% SDS-PAGE
6X His tag antibody [GT161] (GTX628921) dilution: 1:5000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Lodato P.B. et al. FEMS Microbiol Lett 2017; (Epub) Effect of RNase E deficiency on translocon protein synthesis in an RNase E-inducible strain of enterohemorrhagic Escherichia coli O157:H7.
实验应用 : WB
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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