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ABCA1抗体

Orthogonal
Anti-ABCA1 antibody used in Western Blot (WB). GTX27360
Anti-ABCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX27360
Anti-ABCA1 antibody used in Western Blot (WB). GTX27360
Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27360
Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27360
Anti-ABCA1 antibody used in Western Blot (WB). GTX27360
Anti-ABCA1 antibody used in Flow cytometry (FACS). GTX27360

目录号 GTX27360

文献引用 3

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, ChIP assay, Gel supershift assays

种属反应

 Human, Mouse, Rat, Dog, Hamster, Chicken, Pig, Mustelid
产品包装
100 μl (¥6700)
查看所有 ABCA1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500
ICC/IF 1:100
IHC-P 1:200
IHC-Fr Assay dependent
FACS 1:400
IP 1:10 - 1:500
ELISA Assay dependent
ChIP assay Assay dependent
Gel supershift assays Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

254 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Partial peptide sequence (within residues 1100-1300) of human ABCA1 [UniProt# O95477]. Actual immunogen sequence is proprietary information.

纯化方式

Purified by antigen-affinity chromatography

偶联

Unconjugated

RRID

AB_367201

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ATP binding cassette subfamily A member 1 , ABC-1 , ABC1 , CERP , HDLDT1 , TGD

细胞定位

Membrane

功能与背景

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. [provided by RefSeq]

数据库

  • Gene ID: 19 ABCA1
  • UniProt: O95477 ABCA1

研究领域

產品图片

Anti-ABCA1 antibody used in Western Blot (WB). GTX27360

GTX27360 WB Image

WB analysis of mouse peritoneal macrophages using GTX27360 ABCA1 antibody.
Lane 1 : T09 cell lysate
Lane 2 : Induced T09 cell lysate

Anti-ABCA1 antibody used in IHC (Paraffin sections) (IHC-P). GTX27360

GTX27360 IHC-P Image

IHC-P analysis of prostate tissue using GTX27360 ABCA1 antibody.

Anti-ABCA1 antibody used in Western Blot (WB). GTX27360

GTX27360 WB Image

Orthogonal

WB analysis of RAW264.7 cells treated with vehicle (-) or 9-cisretinoic acid and 22rhydroxycholesterol (+) using GTX27360 ABCA1 antibody.
Loading : 40μg

Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27360

GTX27360 ICC/IF Image

ICC/IF analysis of HepG2 cells using GTX27360 ABCA1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml
Fixation : 10% formalin
Permibilization : 0.5% Triton-X100

Anti-ABCA1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX27360

GTX27360 ICC/IF Image

ICC/IF analysis of HepG2 cells(serum starvation 24hrs and treated with 1uM TO9) using GTX27360 ABCA1 antibody.
Green : primary antibody
Red : Tubulin
Blue : DAPI
Dilution : 5 μg/ml
Fixation : 10% formalin
Permibilization : 1X TBS + 0.5% Triton-X100 for 5 minutes

Anti-ABCA1 antibody used in Western Blot (WB). GTX27360

GTX27360 WB Image

WB analysis of post-nuclear lysate of raw macrophages treated with 9-cisretinoic acid and 22R-hydroxycholesterol using GTX27360 ABCA1 antibody.
Loading : 40μg

Anti-ABCA1 antibody used in Flow cytometry (FACS). GTX27360

GTX27360 FACS Image

FACS analysis of HeLa cells using GTX27360 ABCA1 antibody. M1 is defined by unstained cells.
Purple : primary antibody
Dilution : 1:400

文献引用

Application Reference

Wang SM et al. Cell Death Discov 2021; 7 (1) : 8 CCAAT/Enhancer-binding protein delta mediates glioma stem-like cell enrichment and ATP-binding cassette transporter ABCA1 activation for temozolomide resistance in glioblastoma.
实验应用 : WB
种属反应 : Human
Chan HC et al. Biomedicines 2020; 8 (12) An Increased Plasma Level of ApoCIII-Rich Electronegative High-Density Lipoprotein May Contribute to Cognitive Impairment in Alzheimer's Disease.
实验应用 : WB
种属反应 : Mouse
Yeh YT et al. Nutrients 2017; 9 (10) Chinese Olive (Canarium album L.) Fruit Extract Attenuates Metabolic Dysfunction in Diabetic Rats.
实验应用 : WB
种属反应 : Rat
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产品包装 报价 (¥)
¥ 6700
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