GeneTex中国官方网站
  • China

ACE2抗体 [GT19410]

KO/KD Comparison
Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897
Anti-ACE2 antibody [GT19410] used in Lateral Flow (Lateral Flow). GTX635897
Anti-ACE2 antibody [GT19410] used in ELISA (ELISA). GTX635897
Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897
Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897
Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897
Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897
Anti-ACE2 antibody [GT19410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635897

目录号 GTX635897

1

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT19410

同种型

 IgG2b

实验应用

 WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA

种属反应

 Human, Mouse, Monkey
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ACE2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635897 , Detection : GTX628914-01
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

92 kDa. ( Note )

产品说明

This antibody was raised against human ACE2 Extracellular domain.

预测种属反应

Rat, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.55 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human ACE2. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A

偶联

Unconjugated

RRID

AB_2909920

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

angiotensin I converting enzyme 2 , ACEH

细胞定位

Cytoplasm

功能与背景

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897

GTX635897 WB Image

KO/KD

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ACE2 antibody [GT19410] used in Lateral Flow (Lateral Flow). GTX635897

GTX635897 Lateral Flow Image

SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins. The signal intensity of the test line (T) decreases due to competition by the neutralizing antibody, while the control line (C) demonstrates a valid test.

Neutralizing Antibody: Spike RBD antibody [HL1002] (GTX635791)
C Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: Anti-human ACE2 antibody (GTX635897)
T Line:
Detector: Colloidal gold-conjugated human ACE2 (ECO) protein (GTX135683-pro) / Capture: SARS-CoV-2 Spike RBD protein

Anti-ACE2 antibody [GT19410] used in ELISA (ELISA). GTX635897

GTX635897 ELISA Image

Sandwich ELISA detection of recombinant Human ACE2 (ECD) protein, His and Avi tag (active) (GTX01550-pro) using ACE2 antibody [GT19410] (GTX635897) as capture antibody at concentration of 5 μg/mL and 6X His tag antibody [GT359] (HRP) (GTX628914-01) as detection antibody at concentration of 0.1 μg/mL.

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897

GTX635897 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

GTX635897 IHC-P Image

Comparison

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right)

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-ACE2 antibody [GT19410] used in IHC (Paraffin sections) (IHC-P). GTX635897

GTX635897 IHC-P Image

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [GT19410] used in Western Blot (WB). GTX635897

GTX635897 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [GT19410] (GTX635897) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ACE2 antibody [GT19410] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635897

GTX635897 ICC/IF Image

ACE2 antibody [GT19410] detects ACE2 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ACE2 stained by ACE2 antibody [GT19410] (GTX635897) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

文献引用

目前没有使用 ACE2 antibody [GT19410] (GTX635897) 的文献被收录。快来回报我们第一篇参考文献吧！
我要回报文献

产品评论

ACE2 antibody [GT19410] 目录号 GTX635897

Rating
( Average 5 based on 1 users reviews)
实验应用
Lateral Flow(Lateral Flow)
( Average 5 based on 1 users reviews)
Anti-ACE2 antibody [GT19410] used in Lateral Flow (Lateral Flow). GTX635897
Date : Anonymous submitted on 10-Nov-2021
Score :
Application Tested : Lateral Flow
Figure Note : SARS-CoV-2 neutralizing antibody interferes with the binding of ACE2 and spike RBD proteins.
我要评论产品
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com