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ACE2抗体 [HL1092]

Recombinant antibody KO/KD Comparison Orthogonal Overexpression
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265
Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636265
Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265
Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

目录号 GTX636265

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1092

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Cat, Dog, African green monkey
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ACE2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

92 kDa. ( Note )

产品说明

This antibody was raised against human ACE2 Extracellular domain.
我们不建议将此产品应用于 Mouse samples.

预测种属反应

Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human ACE2. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909957

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

angiotensin I converting enzyme 2 , ACEH

细胞定位

Cytoplasm

功能与背景

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265

GTX636265 IHC-P Image

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded cat colon.
ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265

GTX636265 IHC-P Image

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded dog stomach.
ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [HL1092] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636265

GTX636265 ICC/IF Image

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: VeroE6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ACE2 antibody [HL1092] used in IHC (Paraffin sections) (IHC-P). GTX636265

GTX636265 IHC-P Image

Comparison

ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded human kidney.
ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000 and competitor's antibody diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right)

*Competitor's antibody is not affiliated with GeneTex and does not endorse this product.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Various whole cell and tissue extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [HL1092] used in Western Blot (WB). GTX636265

GTX636265 WB Image

Orthogonal

Untreated (–) and treated (+) VeroE6 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
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¥ 1700
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