产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
Assay dependent |
Assay dependent |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human ACE2 Extracellular domain.
我们不建议将此产品应用于 Mouse samples.
预测种属反应
Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the Extracellular domain of human ACE2. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909957
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
angiotensin I converting enzyme 2 , ACEH
细胞定位
Cytoplasm
功能与背景
The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX636265 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX636265 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX636265 IHC-P Image
ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat colon.ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636265 IHC-P Image
ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded dog stomach.ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636265 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636265 ICC/IF Image
ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunofluorescent analysis.Sample: VeroE6 cells were fixed in ice-cold MeOH for 5 min.Green: ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636265 IHC-P Image
ACE2 antibody [HL1092] detects ACE2 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded human kidney.ACE2 stained by ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000 and competitor's antibody diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min (left) and Tris-EDTA buffer, pH 9.0, 15min (right) *Competitor's antibody is not affiliated with GeneTex and does not endorse this product.
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GTX636265 WB Image
Various whole cell and tissue extracts were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.
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GTX636265 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636265 WB Image
Untreated (–) and treated (+) VeroE6 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [HL1092] (GTX636265) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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