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ACE2抗体 [N1N2], N-term

KO/KD Orthogonal Overexpression
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Flow cytometry (FACS). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395
Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

目录号 GTX101395

文献引用 27
4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, FACS, ELISA

种属反应

 Human, Mouse, Rat, African green monkey
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ACE2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
FACS Assay dependent
ELISA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

92 kDa. ( Note )

预测种属反应

Cat, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the Extracellular domain of human ACE2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240451

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

angiotensin I converting enzyme 2 , ACEH

细胞定位

Processed angiotensin-converting enzyme 2: Secreted , Cell membrane

功能与背景

The protein encoded by this gene belongs to the angiotensin-converting enzyme family of dipeptidyl carboxydipeptidases and has considerable homology to human angiotensin 1 converting enzyme. This secreted protein catalyzes the cleavage of angiotensin I into angiotensin 1-9, and angiotensin II into the vasodilator angiotensin 1-7. The organ- and cell-specific expression of this gene suggests that it may play a role in the regulation of cardiovascular and renal function, as well as fertility. In addition, the encoded protein is a functional receptor for the spike glycoprotein of the human coronaviruses SARS and HCoV-NL63. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

KO/KD

Non-transfected (–) and transfected (+) Caco-2 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Orthogonal

Untreated (–) and treated (+) Vero E6 whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395

GTX101395 ELISA Image

Indirect ELISA analysis performed by coating plate with recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag protein (GTX135683-pro) (15.63-1000 ng/mL). Coated protein probed with ACE2 antibody [N1N2], N-term (GTX101395) (0.1 μg/mL). Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded rat lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:2000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

GTX135683-pro Human ACE2 (ECD) protein , mouse IgG Fc tag protein (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane and cytoplasm by immunofluorescent analysis.
Sample: Caco-2 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

KO/KD

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in ELISA (ELISA). GTX101395

GTX101395 ELISA Image

Functional ELISA analysis of immobilized recombinant SARS-CoV-2 (COVID-19) Spike RBD protein, His tag (active) (GTX01546-pro) (coated at 2 μg/mL) binding to soluble recombinant Human ACE2 (ECD) protein , mouse IgG Fc tag (GTX135683-pro) (4.5-10000 ng/mL). Bound protein was detected by ACE2 antibody [N1N2], N-term (GTX101395) (0.5 μg/mL) and Rabbit IgG antibody (HRP) (GTX213110-01) (1:10000) detected bound primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse duodenum.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Western Blot (WB). GTX101395

GTX101395 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunofluorescent analysis.
Sample: Vero E6 cells were fixed in ice-cold MeOH for 5 min.
Green: ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

ACE2 antibody [N1N2], N-term detects ACE2 protein at cell membrane by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
ACE2 stained by ACE2 antibody [N1N2], N-term (GTX101395) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

The data was published in the 2022 in Biomedicines. PMID: 35625919

Anti-ACE2 antibody [N1N2], N-term used in Flow cytometry (FACS). GTX101395

GTX101395 FACS Image

The data was published in the 2022 in Biomedicines. PMID: 35625919

Anti-ACE2 antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX101395

GTX101395 IHC-P Image

The data was published in the 2022 in Biomedicines. PMID: 35625919

Anti-ACE2 antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101395

GTX101395 ICC/IF Image

The data was published in the 2022 in Biomedicines. PMID: 35625919

文献引用

Application Reference

Lin CH et al. Int J Mol Sci 2024; 25 (5) Inhibitory Efficacy of Main Components of Scutellaria baicalensis on the Interaction between Spike Protein of SARS-CoV-2 and Human Angiotensin-Converting Enzyme II.
A Escalera et al. Emerg Microbes Infect 2023; : 2297553 The impact of S2 mutations on Omicron SARS-CoV-2 cell surface expression and fusogenicity
实验应用 : WB
Chen YR et al. Int J Mol Sci 2023; 24 (20) Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro.
Resnick JD et al. bioRxiv 2023; Growth media affects susceptibility of air-lifted human nasal epithelial cell cultures to SARS-CoV2, but not Influenza A, virus infection.
Joh-Sin Wu et al. Viruses 2023; 15 (8) : 1762 Development of Zika Virus Mini-Replicon Based Single-Round Infectious Particles as Gene Delivery Vehicles
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产品评论

ACE2 antibody [N1N2], N-term 目录号 GTX101395

Rating
( Average 5 based on 4 users reviews)
实验应用
Flow cytometry(FACS)
( Average 5 based on 4 users reviews)
Anti-ACE2 antibody [N1N2], N-term used in Flow cytometry (FACS). GTX101395
Date : Anonymous submitted on 30-Jun-2022
Score :
Application Tested : FACS
Sample Species : Hu
Sample : 293T cells with/without Human ACE2 transfection
Blocking : 1% FBS in PBS, 4°C, 0.25Hr
Primary Antibody : 1:250, 4°C, 0.5Hr
Anti-ACE2 antibody [N1N2], N-term used in Flow cytometry (FACS). GTX101395
Date : Anonymous submitted on 13-Oct-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : Human ACE2 transfected 293T cells
Primary Antibody : 1:100, 4°C, 0.5Hr
Figure Note : Alexa 647-anti-Rabbit-IgG was used for secondary antibody to detect ACE2 expression (shown in red). Normal rabbit IgG instead of anti-ACE2 antibody was used as negative control (shown in blue)
Anti-ACE2 antibody [N1N2], N-term used in Flow cytometry (FACS). GTX101395
Date : Anonymous submitted on 22-Mar-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : THP-1 cells
Blocking : 1% FBS in PBS, 4°C, 0.25Hr
Primary Antibody : 1:250, 4°C, 0.5Hr
Anti-ACE2 antibody [N1N2], N-term used in Flow cytometry (FACS). GTX101395
Date : Anonymous submitted on 22-Mar-2020
Score :
Application Tested : FACS
Sample Species : Hu
Sample : K562 cells
Blocking : 1% FBS in PBS, 4°C, 0.25Hr
Primary Antibody : 1:250, 4°C, 0.5Hr
我要评论产品
Application Reference
Lin CH et al. Int J Mol Sci 2024; 25 (5) Inhibitory Efficacy of Main Components of Scutellaria baicalensis on the Interaction between Spike Protein of SARS-CoV-2 and Human Angiotensin-Converting Enzyme II.
A Escalera et al. Emerg Microbes Infect 2023;:2297553 The impact of S2 mutations on Omicron SARS-CoV-2 cell surface expression and fusogenicity
实验应用 : WB
Chen YR et al. Int J Mol Sci 2023; 24 (20) Anisomeles indica Extracts and Their Constituents Suppress the Protein Expression of ACE2 and TMPRSS2 In Vivo and In Vitro.
Resnick JD et al. bioRxiv 2023; Growth media affects susceptibility of air-lifted human nasal epithelial cell cultures to SARS-CoV2, but not Influenza A, virus infection.
Joh-Sin Wu et al. Viruses 2023; 15 (8):1762 Development of Zika Virus Mini-Replicon Based Single-Round Infectious Particles as Gene Delivery Vehicles
Isobe A et al. Commun Biol 2022; 5 (1):1188 ACE2 N-glycosylation modulates interactions with SARS-CoV-2 spike protein in a site-specific manner.
实验应用 : WB
种属反应 : Human
Chien LH et al. Biomed Pharmacother 2022; 153:113434 Study on the potential of Sanghuangporus sanghuang and its components as COVID-19 spike protein receptor binding domain inhibitors.
实验应用 : WB
种属反应 : Human
Sun TK et al. Int J Mol Sci 2022; 23 (23) Schizophyllum commune Reduces Expression of the SARS-CoV-2 Receptors ACE2 and TMPRSS2.
实验应用 : WB
种属反应 : Human
Wang WJ et al. J Food Biochem 2022; 46 (10):e14354 Peimine inhibits variants of SARS-CoV-2 cell entry via blocking the interaction between viral spike protein and ACE2.
Hoai-Nga Thi Nguyen et al. Front Oncol 2022; 12:923467 SARS-CoV-2 M Protein Facilitates Malignant Transformation of Breast Cancer Cells.
实验应用 : FACS
种属反应 : Human
En-Cheng Lin et al. Biomedicines 2022; 10 (5) IL-33 Enhances ACE2 Expression on Epidermal Keratinocytes in Atopic Dermatitis: A Plausible Issue for SARS-CoV-2 Transmission in Inflamed Atopic Skin.
实验应用 : ICC/IF, IHC-P, FACS
种属反应 : Human
Hsin Yeh et al. Phytomedicine 2022; 103:154215 GMI, a protein from Ganoderma microsporum, induces ACE2 degradation to alleviate infection of SARS-CoV-2 Spike-pseudotyped virus.
实验应用 : WB
种属反应 : Human
Zhenfeng Wang et al. Cell Mol Immunol 2022; 19 (2):210-221 SARS-CoV-2 treatment effects induced by ACE2-expressing microparticles are explained by the oxidized cholesterol-increased endosomal pH of alveolar macrophages.
实验应用 : ICC/IF, FACS
种属反应 : Human
Miao-Hsi Hsieh et al. Front Immunol 2021; 12:641360 Human Surfactant Protein D Binds Spike Protein and Acts as an Entry Inhibitor of SARS-CoV-2 Pseudotyped Viral Particles.
实验应用 : FACS
Keiji Tanimoto et al. Sci Rep 2021; 11 (1):16629 Inhibiting SARS-CoV-2 infection in vitro by suppressing its receptor, angiotensin-converting enzyme 2, via aryl-hydrocarbon receptor signal.
实验应用 : WB
Cheng FJ et al. Nutrients 2021; 13 (8) Hesperidin Is a Potential Inhibitor against SARS-CoV-2 Infection.
实验应用 : WB
种属反应 : Human
Melano I et al. Viruses 2021; 13 (7) Effects of Basic Amino Acids and Their Derivatives on SARS-CoV-2 and Influenza-A Virus Infection.
实验应用 : WB
种属反应 : Human
de Vries M et al. bioRxiv 2021; (Epub) A comparative analysis of SARS-CoV-2 antivirals in human airway models characterizes 3CLpro inhibitor PF-00835231 as a potential new treatment for COVID-19.
Khanh VC et al. Stem Cells Dev 2021; 30 (15):758-772 Wharton's Jelly Mesenchymal Stem Cell-Derived Extracellular Vesicles Reduce SARS-CoV2-Induced Inflammatory Cytokines Under High Glucose and Uremic Toxin Conditions.
实验应用 : ICC/IF
种属反应 : Human
Yamada T. et al. Nat Immunol 2021; (Epub) RIG-I triggers a signaling-abortive anti-SARS-CoV-2 defense in human lung cells.
实验应用 : WB
种属反应 : Human
de Vries M et al. J Virol 2021; A comparative analysis of SARS-CoV-2 antivirals characterizes 3CLpro inhibitor PF-00835231 as a potential new treatment for COVID-19.
实验应用 : WB
种属反应 : Human
Tanimoto K. et al. bioRxiv 2021; (Epub) Inhibiting SARS-CoV-2 infection in vitro by suppressing its receptor, angiotensin-converting enzyme 2, via aryl-hydrocarbon receptor signal.
实验应用 : WB
种属反应 : Human
Garc?a-Arroyo FE et al. Biomed Res Int 2020; 2020:4281802 Restricted Water Intake and Hydration with Fructose-Containing Beverages during Infancy Predispose to Aggravate an Acute Renal Ischemic Insult in Adolescent Rats.
实验应用 : WB
种属反应 : Rat
Bessa ASM et al. Hypertens Res 2019; Stimulation of the ACE2/Ang-(1-7)/Mas axis in hypertensive pregnant rats attenuates cardiovascular dysfunction in adult male offspring.
实验应用 : WB
种属反应 : Rat
Queiroz-Junior CM et al. Bone 2019; 128:115041 The angiotensin converting enzyme 2/angiotensin-(1-7)/Mas Receptor axis as a key player in alveolar bone remodeling.
实验应用 : WB
种属反应 : Rat
Lin C.I. et al. Int. J. Biol. Sci. 2018; 14 (3):253-265 Instillation of particulate matter 2.5 induced acute lung injury and attenuated the injury recovery in ACE2 knockout mice.
实验应用 : WB
种属反应 : Mouse
Foureaux G et al. Invest Ophthalmol Vis Sci 2013; 54 (6):4296-306 Antiglaucomatous effects of the activation of intrinsic Angiotensin-converting enzyme 2.
实验应用 : IHC-P
种属反应 : Rat
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Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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