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AChE抗体 [HL1102]

Recombinant antibody Comparison Orthogonal
Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298
Anti-AChE antibody [HL1102] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636298
Anti-AChE antibody [HL1102] used in IHC (Paraffin sections) (IHC-P). GTX636298
Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298
Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298
Anti-AChE antibody [HL1102] used in IHC (Paraffin sections) (IHC-P). GTX636298
Anti-AChE antibody [HL1102] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636298

目录号 GTX636298

1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1102

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat, Cat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 AChE 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

68 kDa. ( Note )

预测种属反应

Bovine, Guinea pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human AChE. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909963

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

acetylcholinesterase (Cartwright blood group) , ACEE , ARACHE , N-ACHE , YT

细胞定位

Cell junction, synapse,Secreted,Cell membrane

功能与背景

Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. [provided by RefSeq, Jul 2008]

数据库

研究领域

產品图片

Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298

GTX636298 WB Image

Comparison

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-AChE antibody [HL1102] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636298

GTX636298 ICC/IF Image

AChE antibody [HL1102] detects AChE protein at dendrite by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AChE antibody [HL1102] used in IHC (Paraffin sections) (IHC-P). GTX636298

GTX636298 IHC-P Image

AChE antibody [HL1102] detects AChE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat brain.
AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298

GTX636298 WB Image

Orthogonal

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298

GTX636298 WB Image

Human tissue extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AChE antibody [HL1102] (GTX636298) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-AChE antibody [HL1102] used in IHC (Paraffin sections) (IHC-P). GTX636298

GTX636298 IHC-P Image

AChE antibody [HL1102] detects AChE protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AChE antibody [HL1102] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636298

GTX636298 ICC/IF Image

AChE antibody [HL1102] detects AChE protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AChE stained by AChE antibody [HL1102] (GTX636298) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

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产品评论

AChE antibody [HL1102] 目录号 GTX636298

Rating
( Average 5 based on 1 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-AChE antibody [HL1102] used in Western Blot (WB). GTX636298
Date : Anonymous submitted on 10-Aug-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : C4-2B cells with/without treatment
Amount used : 60μg
Blocking : 5% milk in PBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 8Hr
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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