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AGTR1抗体 [HL2524]

Recombinant antibody Orthogonal
Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885
Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885
Anti-AGTR1 antibody [HL2524] used in IHC (Paraffin sections) (IHC-P). GTX638885
Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885
Anti-AGTR1 antibody [HL2524] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638885
Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

目录号 GTX638885

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL2524

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB Assay dependent
ICC/IF Assay dependent
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

41 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Synthetic peptide of human AGTR1

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

angiotensin II receptor type 1 , AG2S , AGTR1B , AT1 , AT1AR , AT1B , AT1BR , AT1R , AT2R1 , HAT1R

细胞定位

Cell membrane; Multi-pass membrane protein

功能与背景

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene. [provided by RefSeq, Jul 2012]

数据库

研究领域

產品图片

Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

GTX638885 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

GTX638885 WB Image

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-AGTR1 antibody [HL2524] used in IHC (Paraffin sections) (IHC-P). GTX638885

GTX638885 IHC-P Image

AGTR1 antibody [HL2524] detects AGTR1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded Huh-7 xenograft.
AGTR1 stained by AGTR1 antibody [HL2524] (GTX638885) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

GTX638885 WB Image

Orthogonal

Huh-7 cytoplasma extracts and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. (CE: cytoplasma extract; ME: membrane extract)

Anti-AGTR1 antibody [HL2524] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX638885

GTX638885 ICC/IF Image

AGTR1 antibody [HL2524] detects AGTR1 protein at cell membrane by immunofluorescent analysis.
Sample: Huh-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AGTR1 stained by AGTR1 antibody [HL2524] (GTX638885) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-AGTR1 antibody [HL2524] used in Western Blot (WB). GTX638885

GTX638885 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AGTR1 antibody [HL2524] (GTX638885) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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