Wild-type (WT) and AKT1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 | 10 | 5 | 50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

AKT1 antibody [HL1142] detects AKT1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AKT1 antibody [HL1142] detects AKT1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

AKT1 antibody [HL1142] detects AKT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded cat mammary gland.
AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

AKT1 antibody [HL1142] detects AKT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded dog mammary gland.
AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.