产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:1000-1:10000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Mouse, Zebrafish, Sheep, Bovine, Chicken, Pig, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human AKT1. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2909978
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
AKT serine/threonine kinase 1 , AKT , CWS6 , PKB , PKB-ALPHA , PRKBA , RAC , RAC-ALPHA
细胞定位
Cytoplasm,Nucleus,Cell membrane
功能与背景
The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Mutations in this gene have been associated with the Proteus syndrome. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2011]
数据库
研究领域
產品图片
![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/templates/default/js/lightbox/images/loading.gif "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
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GTX636413 WB Image
Wild-type (WT) and AKT1 knockout (KO) HeLa cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636413 ICC/IF Image
AKT1 antibody [HL1142] detects AKT1 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX636413 IHC-P Image
AKT1 antibody [HL1142] detects AKT1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636413 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 | 10 | 5 | 50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636413 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
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GTX636413 WB Image
Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with AKT1 antibody [HL1142] (GTX636413) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX636413 IHC-P Image
AKT1 antibody [HL1142] detects AKT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded dog mammary gland.AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX636413 IHC-P Image
AKT1 antibody [HL1142] detects AKT1 protein at cell membrane and cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded cat mammary gland.AKT1 stained by AKT1 antibody [HL1142] (GTX636413) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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产品评论
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![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/upload/website/prouct_img/small/GTX636413/GTX636413_44501_20220211_WB_KO_watermark_w_23061202_912.webp "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636413](/upload/website/prouct_img/small/GTX636413/GTX636413_T-44410_20211008_ICC_IF_w_23061202_142.webp "Anti-AKT1 antibody [HL1142] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636413")
![Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413](/upload/website/prouct_img/small/GTX636413/GTX636413_44501_20211210_IHC-P_w_23061202_342.webp "Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/upload/website/prouct_img/small/GTX636413/GTX636413_44501_20211210_WB_B_w_23061202_732.webp "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/upload/website/prouct_img/small/GTX636413/GTX636413_44501_20220506_WB_R_w_23061202_672.webp "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/upload/website/prouct_img/small/GTX636413/GTX636413_44501_20221209_WB_D_22121123_803.webp "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
![Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413](/upload/website/prouct_img/small/GTX636413/GTX636413_44501_20230203_IHC-P_Dog_23020621_769.webp "Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413")
![Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413](/upload/website/prouct_img/small/GTX636413/GTX636413_44501_20230203_IHC-P_Cat_23020621_786.webp "Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/upload/website/prouct_img/normal/GTX636413/GTX636413_44501_20220211_WB_KO_watermark_w_23061202_912.webp "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636413](/upload/website/prouct_img/normal/GTX636413/GTX636413_T-44410_20211008_ICC_IF_w_23061202_142.webp "Anti-AKT1 antibody [HL1142] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636413")
![Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413](/upload/website/prouct_img/normal/GTX636413/GTX636413_44501_20211210_IHC-P_w_23061202_342.webp "Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/upload/website/prouct_img/normal/GTX636413/GTX636413_44501_20211210_WB_B_w_23061202_732.webp "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/upload/website/prouct_img/normal/GTX636413/GTX636413_44501_20220506_WB_R_w_23061202_672.webp "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
![Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413](/upload/website/prouct_img/normal/GTX636413/GTX636413_44501_20221209_WB_D_22121123_803.webp "Anti-AKT1 antibody [HL1142] used in Western Blot (WB). GTX636413")
![Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413](/upload/website/prouct_img/normal/GTX636413/GTX636413_44501_20230203_IHC-P_Dog_23020621_769.webp "Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413")
![Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413](/upload/website/prouct_img/normal/GTX636413/GTX636413_44501_20230203_IHC-P_Cat_23020621_786.webp "Anti-AKT1 antibody [HL1142] used in IHC (Paraffin sections) (IHC-P). GTX636413")
