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ALDH2抗体

KO/KD Orthogonal
Anti-ALDH2 antibody used in IHC (Whole mount) (IHC-Wm). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429
Anti-ALDH2 antibody used in ELISA (ELISA). GTX101429
Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

目录号 GTX101429

文献引用 7
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, IHC-Wm, ELISA, IHC, Sandwich ELISA

种属反应

 Human, Mouse, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 ALDH2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Wm Assay dependent
ELISA 1:1000-1:10000
IHC Assay dependent
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture: GTX101429 , Detection: GTX88073
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

56 kDa. ( Note )

阳性对照

GL261 , C8D30 , BCL-1 , Raw264.7

预测种属反应

Rat, Bovine, Pig, Xenopus tropicalis(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.23 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ALDH2. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949628

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

aldehyde dehydrogenase 2 family member , ALDH-E2 , ALDHI , ALDM

细胞定位

Mitochondrion matrix

功能与背景

This protein belongs to the aldehyde dehydrogenase family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme. This gene encodes a mitochondrial isoform, which has a low Km for acetaldehydes, and is localized in mitochondrial matrix. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-ALDH2 antibody used in IHC (Whole mount) (IHC-Wm). GTX101429

GTX101429 IHC-Wm Image

ALDH2 antibody detects ALDH2 protein at mitochondria on whole-mount zebrafish embryos by immunohistochemical analysis.
Sample: Paraformaldehyde-fixed zebrafish embryos.
ALDH2 antibody (GTX101429) dilution: 1:200.
Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

ALDH2 antibody detects ALDH2 protein by Western blot analysis. Zebrafish tissue extracts (30 μg) was separated by 10 % SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) at a dilution of 1:2000.

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using ALDH2 antibody (GTX101429) at 1:300 dilution.

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

ALDH2 antibody detects ALDH2 protein at mitochondria on mouse colon by immunohistochemical analysis.
Sample: Paraffin-embedded mouse colon.
ALDH2 antibody (GTX101429) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

ALDH2 antibody detects ALDH2 protein by Western blot analysis. Whole cell extracts (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) at a dilution of 1:500.

Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

Orthogonal

HepG2 whole cell and cytoplasm extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ALDH2 antibody (GTX101429) diluted at 1:500.

Anti-ALDH2 antibody used in Western Blot (WB). GTX101429

GTX101429 WB Image

ALDH2 antibody detects ALDH2 protein by western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg BCL-1 whole cell lysae/extract
D. 30 μg Raw264.7 whole cell lysate/extract
7.5% SDS-PAGE
ALDH2 antibody (GTX101429) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

ALDH2 antibody detects ALDH2 protein at mitochondria on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
ALDH2 antibody (GTX101429) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ALDH2 antibody used in ELISA (ELISA). GTX101429

GTX101429 ELISA Image

ELISA detection of ALDH2 using GTX101429 for capture at a concentration of 2.5 μg/mL and GTX88073 for detection at a concentration of 1.5 μg/mL.

Anti-ALDH2 antibody used in IHC (Paraffin sections) (IHC-P). GTX101429

GTX101429 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using ALDH2(GTX101429) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

文献引用

Application Reference

Lin YH et al. J Cancer 2023; 14 (10) : 1689-1706 Analysis of Aldehyde Dehydrogenase 2 as a Prognostic Marker Associated with Immune Cell infiltration and Chemotherapy Efficacy in Head and Neck Squamous Cell Carcinoma.
Yu-Hsuan Lin et al. J. Cancer 2023; 14 (10) : 1689-1706 Analysis of Aldehyde Dehydrogenase 2 as a Prognostic Marker Associated with Immune Cell infiltration and Chemotherapy Efficacy in Head and Neck Squamous Cell Carcinoma
实验应用 : WB, IHC
种属反应 : Human
Masato A et al. NPJ Parkinsons Dis 2023; 9 (1) : 42 DOPAL initiates αSynuclein-dependent impaired proteostasis and degeneration of neuronal projections in Parkinson's disease.
实验应用 : WB
Shiau-Mei Chen et al. Biomolecules 2021; 11 (10) Activation of Aldehyde Dehydrogenase 2 Ameliorates Glucolipotoxicity of Pancreatic Beta Cells.
实验应用 : WB
种属反应 : Mouse
Kitakaze T et al. Arch Biochem Biophys 2020; 686 : 108329 6-(Methylsulfinyl)hexyl isothiocyanate protects acetaldehyde-caused cytotoxicity through the induction of aldehyde dehydrogenase in hepatocytes.
实验应用 : WB
种属反应 : Mouse
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产品评论

ALDH2 antibody 目录号 GTX101429

Rating
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实验应用
Western Blot(WB)
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Anti-ALDH2 antibody used in Western Blot (WB). GTX101429
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : MIN6 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, 16Hr
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SDS
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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