产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Mouse brain , Raji
产品说明
Based on sequence homology, this antibody may cross react with ANP32B or ANP32C proteins.
预测种属反应
Rat, Zebrafish, Bovine, Dog, Xenopus tropicalis, Rhesus Monkey(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 1% BSA, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human ANP32A. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_10624431
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
acidic nuclear phosphoprotein 32 family member A , C15orf1 , HPPCn , I1PP2A , LANP , MAPM , PHAP1 , PHAPI , PP32
细胞定位
Nucleus , Cytoplasm
功能与背景
Implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. Plays a role in E4F1-mediated transcriptional repression.
数据库
研究领域
產品图片
![Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933](/templates/default/js/lightbox/images/loading.gif "Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933")
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GTX115933 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed A431, using ANP32A(GTX115933) antibody at 1:200 dilution.
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GTX115933 IHC-P Image
ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
ANP32A antibody [N1C2] (GTX115933) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX115933 WB Image
Sample (30 ug of whole cell lysate)
A: Raji
12% SDS PAGE
GTX115933 diluted at 1:1000
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GTX115933 WB Image
Sample (50 ug of whole cell lysate)
A: mouse brain
12% SDS PAGE
GTX115933 diluted at 1:1000
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GTX115933 IHC-P Image
ANP32A antibody [N1C2] detects ANP32A protein at nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
ANP32A antibody [N1C2] (GTX115933) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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文献引用
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产品评论
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![Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_40289_IFA_w_23060519_522.webp "Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933")
![Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_40289_20150714_IHC-P_M_w_23060519_365.webp "Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_WB_w_23060519_440.webp "Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_40289_WB_M_brain_w_23060519_590.webp "Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933")
![Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933](/upload/website/prouct_img/small/GTX115933/GTX115933_40289_20150714_IHC-P_M_2_w_23060519_147.webp "Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_40289_IFA_w_23060519_522.webp "Anti-ANP32A antibody [N1C2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115933")
![Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_40289_20150714_IHC-P_M_w_23060519_365.webp "Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_WB_w_23060519_440.webp "Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933")
![Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_40289_WB_M_brain_w_23060519_590.webp "Anti-ANP32A antibody [N1C2] used in Western Blot (WB). GTX115933")
![Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933](/upload/website/prouct_img/normal/GTX115933/GTX115933_40289_20150714_IHC-P_M_2_w_23060519_147.webp "Anti-ANP32A antibody [N1C2] used in IHC (Paraffin sections) (IHC-P). GTX115933")
