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APC抗体 [C3], C-term

Anti-APC antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116009
Anti-APC antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116009
Anti-APC antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116009
Anti-APC antibody [C3], C-term used in Western Blot (WB). GTX116009
Anti-APC antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116009

目录号 GTX116009

文献引用 3
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 APC 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

312 kDa. ( Note )

预测种属反应

Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.44 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human APC. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_10727214

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

APC regulator of WNT signaling pathway , BTPS2 , DP2 , DP2.5 , DP3 , GS , PPP1R46

功能与背景

This gene encodes a tumor suppressor protein that acts as an antagonist of the Wnt signaling pathway. It is also involved in other processes including cell migration and adhesion, transcriptional activation, and apoptosis. Defects in this gene cause familial adenomatous polyposis (FAP), an autosomal dominant pre-malignant disease that usually progresses to malignancy. Disease-associated mutations tend to be clustered in a small region designated the mutation cluster region (MCR) and result in a truncated protein product. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-APC antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116009

GTX116009 ICC/IF Image

APC antibody [C3], C-term detects APC protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APC protein stained by APC antibody [C3], C-term (GTX116009) diluted at 1:1000.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
Anti-APC antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116009

GTX116009 IHC-P Image

APC antibody [C3], C-term detects APC protein at cytoplasm by immunohistochemical analysis (Autostainer Formulated).
Sample: Paraffin-embedded human colon cancer.
APC stained by APC antibody [C3], C-term (GTX116009) diluted at 1:500.
Antigen Retrieval: Citrate buffer, 20 min

Anti-APC antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX116009

GTX116009 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using APC(GTX116009) antibody at 1:500 dilution.

Anti-APC antibody [C3], C-term used in Western Blot (WB). GTX116009

GTX116009 WB Image

Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with APC antibody [C3], C-term (GTX116009) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-APC antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116009

GTX116009 IHC-P Image

APC antibody [C3], C-term detects APC protein at cytoplasm in rat colon by immunohistochemical analysis.
Sample: Paraffin-embedded rat colon.
APC antibody [C3], C-term (GTX116009) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Elisa Degl'Innocenti et al. Front Cell Neurosci 2022; 16 : 858347 Centrin 2: A Novel Marker of Mature and Neoplastic Human Astrocytes.
实验应用 : IHC-P
种属反应 : Human
Yamaguchi S et al. Oncotarget 2018; 9 (12) : 10818-10827 Identification and characterization of a novel adenomatous polyposis coli mutation in adult pancreatoblastoma.
Moore S et al. Neuroscience 2013; 245 : 166-78 Sex chromosome complement influences functional callosal myelination.
实验应用 : IHC
种属反应 : Mouse
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产品评论

APC antibody [C3], C-term 目录号 GTX116009

Rating
( Average 5 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-APC antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX116009
Date : Anonymous submitted on 21-Oct-2020
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : colon cancer tissue
Fixation : Citrate buffer, 0.3°C
Primary Antibody : 1:500, 4°C, 16Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
Currently in production
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