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APP抗体 [C2C3], C-term

Anti-APP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101336
Anti-APP antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101336
Anti-APP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101336
Anti-APP antibody [C2C3], C-term used in Western Blot (WB). GTX101336
Anti-APP antibody [C2C3], C-term used in Western Blot (WB). GTX101336

目录号 GTX101336

文献引用 6
2

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 APP 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

87 kDa. ( Note )

阳性对照

HepG2 , U87-MG , SK-N-SH , IMR32 , SK-N-AS

预测种属反应

Rabbit, Bovine, Dog, Chicken, Pig, Xenopus tropicalis, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 10% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the C-terminus region of human APP. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1240465

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

amyloid beta precursor protein , AAA , ABETA , ABPP , AD1 , APPI , CTFgamma , CVAP , PN-II , PN2 , preA4

细胞定位

Membrane , Membrane , clathrin-coated pit

功能与背景

This gene encodes a cell surface receptor and transmembrane precursor protein that is cleaved by secretases to form a number of peptides. Some of these peptides are secreted and can bind to the acetyltransferase complex APBB1/TIP60 to promote transcriptional activation, while others form the protein basis of the amyloid plaques found in the brains of patients with Alzheimer disease. Mutations in this gene have been implicated in autosomal dominant Alzheimer disease and cerebroarterial amyloidosis (cerebral amyloid angiopathy). Multiple transcript variants encoding several different isoforms have been found for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-APP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101336

GTX101336 IHC-P Image

APP antibody [C2C3], C-term detects APP protein at cytoplasm in Rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded Rat brain.
APP antibody [C2C3], C-term (GTX101336) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-APP antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101336

GTX101336 ICC/IF Image

APP antibody [C2C3], C-term detects APP protein at cytoplasm by immunofluorescent analysis.
Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: APP protein stained by APP antibody [C2C3], C-term (GTX101336) diluted at 1:400.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-APP antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX101336

GTX101336 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human tonsil, plasma cells, using APP(GTX101336) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-APP antibody [C2C3], C-term used in Western Blot (WB). GTX101336

GTX101336 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with APP antibody [C2C3], C-term (GTX101336) diluted at 1:500.

Anti-APP antibody [C2C3], C-term used in Western Blot (WB). GTX101336

GTX101336 WB Image

Sample(30 μg of whole cell lysate)
A:Hep G2(GTX27900)
7.5% SDS PAGE
GTX101336 diluted at 1:1500

文献引用

Application Reference

Bai N et al. Mol Metab 2022; 63 : 101531 CLSTN3 gene variant associates with obesity risk and contributes to dysfunction in white adipose tissue.
实验应用 : WB
Popov S et al. Proc Natl Acad Sci U S A 2018; (Epub) HIV-1 gag recruits PACSIN2 to promote virus spreading.
实验应用 : WB
种属反应 : Human
Lin S et al. Neurosci Lett 2017; (Epub) Upregulated BMP6 pathway involved in the pathogenesis of Aβ toxicity in vivo.
实验应用 : IHC-P
Meng P et al. Neurosci Res 2015; 94 (Epub) : 43109 Carnosic acid attenuates apoptosis induced by amyloid-β 1-42 or 1-43 in SH-SY5Y human neuroblastoma cells.
实验应用 : WB
种属反应 : Human
Meng P et al. Neurosci Res 2013; 75 (2) : 94-102 Carnosic acid suppresses the production of amyloid-β 1-42 by inducing the metalloprotease gene TACE/ADAM17 in SH-SY5Y human neuroblastoma cells.
实验应用 : WB
种属反应 : Human
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产品评论

APP antibody [C2C3], C-term 目录号 GTX101336

Rating
( Average 5 based on 2 users reviews)
实验应用
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Frozen sections)(IHC-Fr)
( Average 5 based on 1 users reviews)
Anti-APP antibody [C2C3], C-term used in IHC (Frozen sections) (IHC-Fr). GTX101336
Date : Anonymous submitted on 10-Sep-2021
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : hippocampus
Blocking : 1% BSA in PBST, RT, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 1% Triton and Citrate Buffer
Primary Antibody : 1:100, 4°C, > 12Hr
Anti-APP antibody [C2C3], C-term used in Western Blot (WB). GTX101336
Date : Beished Shabangu submitted on 30-Oct-2019
Score :
Application Tested : WB
Sample Species : Ms
Sample : N2a cells
Blocking : 5% Milk, 25°C, 1Hr
Primary Antibody : 1:3000, 4°C, 1Hr
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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