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ASFV p54抗体 [GT1075]

Anti-ASFV p54 antibody [GT1075] used in Western Blot (WB). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635691
Anti-ASFV p54 antibody [GT1075] used in IHC (Paraffin sections) (IHC-P). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in ELISA (ELISA). GTX635691
Anti-ASFV p54 antibody [GT1075] used in ELISA (ELISA). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691
Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

目录号 GTX635691

宿主

Mouse

克隆

Monoclonal

克隆编号

GT1075

同种型

IgG1

实验应用

WB, ICC/IF, IHC-P, ELISA, Lateral Flow, Sandwich ELISA

种属反应

African swine fever virus
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
ICC/IF Assay dependent
IHC-P Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Lateral Flow
Capture : GTX636701, Detection : GTX635691 or Capture : GTX635691, Detection : GTX636562 / GTX636703

Sandwich ELISA
Capture : GTX635690, Detection : GTX635691 or Capture : GTX635691, Detection : GTX135175.
Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.

以下为常规应用缩写的中文注解
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

20 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

保存剂

No Preservative

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

African swine fever virus

免疫原

Recombinant protein encompassing a sequence within the center region of ASFV p54 (ASFV (strain: BA71V)). The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888563

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

African swine fever virus p54

研究领域

產品图片

Anti-ASFV p54 antibody [GT1075] used in Western Blot (WB). GTX635691

GTX635691 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ASFV p54 antibody [GT1075] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635691

GTX635691 ICC/IF Image

ASFV p54 antibody [GT1075] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ASFV p54 antibody [GT1075] used in IHC (Paraffin sections) (IHC-P). GTX635691

GTX635691 IHC-P Image

ASFV p54 antibody [GT1075] detects ASFV p54 protein at cytoplasm by immunohistochemical analysis.
Sample: African swine fever virus envelope protein p54 transfected 293T cell FFPE Cell Pellet Block.
Green: ASFV p54 stained by ASFV p54 antibody [GT1075] (GTX635691) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro, 0.2 μg/ml) by lateral flow assay.

Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 μg/ml). Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 μg/ml).

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635691 [GT1075])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of E.coli expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody [HL1287] (GTX636701)
Detection: ASFV p54 antibody [GT1075] (GTX635691)

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX635691 [GT1075])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (40 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX635691 [GT1075])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of E.coli expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody [HL1287] (GTX636701)
Detection: ASFV p54 antibody [GT1075] (GTX635691)

Anti-ASFV p54 antibody [GT1075] used in ELISA (ELISA). GTX635691

GTX635691 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture:
ASFV p54 antibody [GT1075] (GTX635691) (5 μg/mL)
Detection:
ASFV p54 antibody (GTX135175) (1 μg/mL)

Anti-ASFV p54 antibody [GT1075] used in ELISA (ELISA). GTX635691

GTX635691 ELISA Image

Sandwich ELISA detection of recombinant ASFV p54 using antibodies as below.

Capture:
ASFV p54 antibody [GT853] (GTX635690) (5 μg/mL)
Detection:
HRP-conjugated ASFV p54 antibody [GT1075] (GTX635691) (1 μg/mL)
Please notice that GTX635691 needs to be conjugated to HRP to function as the detection antibody when paired with GTX635690. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro) by lateral flow assay using the monoclonal antibody pair.

Capture : ASFV p54 antibody [HL1287] (GTX636701) Detection : ASFV p54 antibody [GT1075] (GTX635691)

Anti-ASFV p54 antibody [GT1075] used in Lateral Flow (Lateral Flow). GTX635691

GTX635691 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro) by lateral flow assay using the monoclonal antibody pair.

Capture : ASFV p54 antibody [HL1289] (GTX636703) Detection : ASFV p54 antibody [GT1075] (GTX635691)

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产品评论

ASFV p54 antibody [GT1075] 目录号 GTX635691

Rating ( Average 5 based on 2 users reviews)
实验应用
Lateral Flow(Lateral Flow) ( Average 5 based on 2 users reviews)
Date : Anonymous submitted on 21-Apr-2022
Score :
Application Tested : Lateral Flow
Sample Species : ASFV
Sample : 1ng African swine fever virus p54 protein, GTX135175-pro
Figure Note : Detection of African swine fever virus p54 protein (partial) by lateral flow assay using the monoclonal antibody pair.
Capture : ASFV p54 antibody [HL1287] (GTX636701) or ASFV p54 antibody [HL1289] (GTX636703)
Detection : ASFV p54 antibody [GT1075] (GTX635691)
Date : Anonymous submitted on 02-Sep-2021
Score :
Application Tested : Lateral Flow
Sample Species : ASFV
Sample : ASFV p54 protein
Amount used : 0.2 μg/ml
Figure Note : Detection of ASFV p54 protein (0.2 μg/ml) by lateral flow assay.
Capture : Biotin-labeled ASFV p54 antibody [GT1075] (3.32 μg/ml).
Detection : FITC-labeled ASFV p54 antibody [GT853] (3.32 μg/ml).
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
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