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ASFV p54抗体 [HL1287]

Recombinant antibody
Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701
Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701
Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Western Blot (WB). GTX636701
Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636701
Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

目录号 GTX636701

宿主

Rabbit

克隆

Monoclonal

克隆编号

HL1287

同种型

IgG

实验应用

WB, ICC/IF, ELISA, Lateral Flow, Sandwich ELISA

种属反应

African swine fever virus
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:100000
ICC/IF Assay dependent
ELISA Assay dependent
Lateral Flow Assay dependent
Sandwich ELISA Assay dependent

Note :

Lateral Flow
Capture : GTX636701, Detection : GTX635691 / GTX636562 / GTX636703

Sandwich ELISA
Capture : GTX636701, Detection: GTX636703 / GTX636700 or Capture : GTX636703 / GTX636700, Detection: GTX636701.
Please notice that detedtion antibody needs to be conjugated to HRP to function as the detection antibody when paired with capture antibody. Please contact us for custom HRP-conjugated antibody.

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

African swine fever virus

免疫原

Recombinant protein encompassing a sequence within the center region of ASFV p54. (African swine fever virus (strain: BA71V))

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2910049

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

產品图片

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1286] (GTX636700) (1 μg/mL)
Please notice that GTX636700 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1287] (GTX636701) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1289] (GTX636703) (1 μg/mL)
Please notice that GTX636703 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636701. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1286] (GTX636700) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636700. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1287] used in Western Blot (WB). GTX636701

GTX636701 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with ASFV p54 antibody [HL1287] (GTX636701) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASFV p54 antibody [HL1287] used in ELISA (ELISA). GTX636701

GTX636701 ELISA Image

Sandwich ELISA detection of recombinant recombinant HEK293 expressed, full-length ASFV p54 using antibodies as below.

Capture: ASFV p54 antibody [HL1289] (GTX636703) (5 μg/mL)
Detection: HRP-conjugated ASFV p54 antibody [HL1287] (GTX636701) (1 μg/mL)
Please notice that GTX636701 needs to be conjugated to HRP to function as the detection antibody when paired with GTX636703. Please contact us for custom HRP-conjugated antibody.

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX636703 [HL1289])

Samples (20 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of E.coli expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody [HL1287] (GTX636701)
Detection: ASFV p54 antibody [GT1075] (GTX635691)

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX636562 [HL1218])

Samples (10 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of HEK293 expressed, ASFV p54 (ECD) protein by lateral flow assay using the monoclonal antibody pair.

Capture: ASFV p54 antibody (GTX636701 [HL1287])
Detection: ASFV p54 antibody (GTX635691 [GT1075])

Samples (80 ng) :
1. ASFV p54 (ECD) protein (GTX137950-pro)
2. Lysis buffer

Anti-ASFV p54 antibody [HL1287] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636701

GTX636701 ICC/IF Image

ASFV p54 antibody [HL1287] detects ASFV p54 protein by immunofluorescent analysis.
Sample: Mock and transfected 293T cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASFV p54 stained by ASFV p54 antibody [HL1287] (GTX636701) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ASFV p54 antibody [HL1287] used in Lateral Flow (Lateral Flow). GTX636701

GTX636701 Lateral Flow Image

Detection of ASFV p54 (ECD) protein, His Tag (GTX135175-pro) by lateral flow assay using the monoclonal antibody pair.

Capture : ) ASFV p54 antibody [HL1287] (GTX636701) Detection : ASFV p54 antibody [GT1075] (GTX635691)

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产品评论

ASFV p54 antibody [HL1287] 目录号 GTX636701

Rating ( Average 5 based on 2 users reviews)
实验应用
Immunocytochemistry/ Immunofluorescence(ICC/IF) ( Average 5 based on 1 users reviews)
Lateral Flow(Lateral Flow) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 11-Jul-2023
Score :
Application Tested : ICC/IF
Sample : ASFV p54 protein expressed in Vero cells
Fixation : 4% PFA in PBS, RT/20min
Permeabilization /Antigen retrieval : 0.2% Triton X-100 in PBS, RT/0.5hr
Primary Antibody : 1:10000, 4°C, 12Hr
Date : Anonymous submitted on 21-Apr-2022
Score :
Application Tested : Lateral Flow
Sample Species : ASFV
Sample : ASFV p54 recombinant protein, partial
Amount used : 1ng
Figure Note : Detection of African swine fever virus p54 protein (partial) by lateral flow assay using the monoclonal antibody pair.
Capture : ASFV p54 antibody [HL1287] (GTX636701) or ASFV p54 antibody [HL1289] (GTX636703)
Detection : ASFV p54 antibody [GT1075] (GTX635691)
产品包装 报价 (¥)
¥ 4000
¥ 1700
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