产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7
预测种属反应
Rat, Bovine(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.78 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human ASL. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036281
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
argininosuccinate lyase , ASAL
功能与背景
This gene encodes a member of the lyase 1 family. The encoded protein forms a cytosolic homotetramer and primarily catalyzes the reversible hydrolytic cleavage of argininosuccinate into arginine and fumarate, an essential step in the liver in detoxifying ammonia via the urea cycle. Mutations in this gene result in the autosomal recessive disorder argininosuccinic aciduria, or argininosuccinic acid lyase deficiency. A nontranscribed pseudogene is also located on the long arm of chromosome 22. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX113629 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ASL antibody (GTX113629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX113629 WB Image
ASL antibody detects ASL protein by western blot analysis.
A. 30 μg Neuro2A whole lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
10% SDS-PAGE
ASL antibody (GTX113629) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX113629 IHC-P Image
Immunohistochemical analysis of paraffin-embedded U87 xenograft, using ASL(GTX113629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113629 ICC/IF Image
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Argininosuccinate Lyase(GTX113629) antibody at 1:200 dilution.
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GTX113629 IHC-P Image
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ASL(GTX113629) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX113629 IHC-P Image
ASL antibody detects ASL protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded human hepatocellular carcinoma.ASL stained by ASL antibody (GTX113629) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX113629 ICC/IF Image
ASL antibody detects ASL protein at cytoplasm by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ASL protein stained by ASL antibody (GTX113629) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Hoechst 33342 staining.
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