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ASXL1抗体

KO/KD Overexpression
Anti-ASXL1 antibody used in Western Blot (WB). GTX127284
Anti-ASXL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127284
Anti-ASXL1 antibody used in Western Blot (WB). GTX127284

目录号 GTX127284

文献引用 3
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000-1:10000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

165 kDa. ( Note )

阳性对照

GFP-human ASXL1-transfected 293T cells

产品说明

Knockdown/Knockout validation was supported by references (PMID:26385183)

预测种属反应

Zebrafish(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the N-terminus region of human ASXL1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885631

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ASXL transcriptional regulator 1 , BOPS , MDS

细胞定位

Nucleus

功能与背景

This gene is similar to the Drosophila additional sex combs gene, which encodes a chromatin-binding protein required for normal determination of segment identity in the developing embryo. The protein is a member of the Polycomb group of proteins, which are necessary for the maintenance of stable repression of homeotic and other loci. The protein is thought to disrupt chromatin in localized areas, enhancing transcription of certain genes while repressing the transcription of other genes. The protein encoded by this gene functions as a ligand-dependent co-activator for retinoic acid receptor in cooperation with nuclear receptor coactivator 1. Mutations in this gene are associated with myelodysplastic syndromes and chronic myelomonocytic leukemia. Alternative splicing results in multiple transcript variants. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-ASXL1 antibody used in Western Blot (WB). GTX127284

GTX127284 WB Image

Overexpression

ASXL1 antibody detects ASXL1 protein by western blot analysis.
A. 50 μg 293T whole cell lysate/extract
B. 50 μg whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells
ASXL1 antibody (GTX127284) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ASXL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX127284

GTX127284 IHC-P Image

ASXL1 antibody detects ASXL1 protein at nucleus in human esophagus carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus carcinoma.
ASXL1 antibody (GTX127284) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-ASXL1 antibody used in Western Blot (WB). GTX127284

GTX127284 WB Image

Whole cell extract (30 Aμg) was separated by 5% SDS-PAGE, and the membrane was blotted with ASXL1 antibody (GTX127284) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Moon S et al. Cell Death Dis 2018; 9 (11) : 1118 Asxl1 exerts an antiproliferative effect on mouse lung maturation via epigenetic repression of the E2f1-Nmyc axis.
实验应用 : WB
种属反应 : Mouse
Youn HS et al. Sci Rep 2017; 7 (1) : 5198 Asxl1 deficiency in embryonic fibroblasts leads to cellular senescence via impairment of the AKT-E2F pathway and Ezh2 inactivation.
实验应用 : WB
种属反应 : Mouse
Moon S et al. Biochem Biophys Res Commun 2015; 466 (3) : 560-6 Role of Asxl1 in kidney podocyte development via its interaction with Wtip.
实验应用 : WB
种属反应 : Mouse
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产品评论

ASXL1 antibody 目录号 GTX127284

Rating
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实验应用
Western Blot(WB)
( Average 4 based on 1 users reviews)
Anti-ASXL1 antibody used in Western Blot (WB). GTX127284
Date : Anonymous submitted on 17-Oct-2012
Score :
Application Tested : WB
Sample Species : Hu
Sample : 293T cells
Amount used : 50μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:5000, 4°C, 16Hr
条带说明 : A.50ug 293T whole cell lysate/extract, B.50ug whole cell lysate/extract of GFP-human ASXL1-transfected 293T cells
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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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