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ATG12抗体 [GT166]

KO/KD Orthogonal
Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815
Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815
Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815
Anti-ATG12 antibody [GT166] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629815

目录号 GTX629815

文献引用 4

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT166

同种型

 IgG2b

实验应用

 WB, ICC/IF, PLA

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
PLA Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

15 kDa. ( Note )

阳性对照

293T , A431 , HeLa , HepG2 , Neuro2A , GL261 , C8D30 , NIH-3T3 , BCL-1 , Raw264.7 , C2C12

预测种属反应

Pig, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.

纯化方式

Affinity purified by Protein G.

偶联

Unconjugated

RRID

AB_2888163

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

细胞定位

Cytoplasm

功能与背景

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

数据库

研究领域

產品图片

Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815

GTX629815 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody [GT166] (GTX629815) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815

GTX629815 WB Image

ATG12 antibody [GT166] detects ATG12 protein by western blot analysis.
A. 30 μg Neuro2A whole cell lysate/extract
B. 30 μg GL261 whole cell lysate/extract
C. 30 μg C8D30 whole cell lysate/extract
D. 30 μg NIH-3T3 whole cell lysate/extract
E. 30 μg BCL-1 whole cell lysate/extract
F. 30 μg Raw264.7 whole cell lysate/extract
G. 30 μg C2C12 whole cell lysate/extract
12% SDS-PAGE
ATG12 antibody [GT166] (GTX629815) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATG12 antibody [GT166] used in Western Blot (WB). GTX629815

GTX629815 WB Image

ATG12 antibody [GT166] detects ATG12 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
12% SDS-PAGE
ATG12 antibody [GT166] (GTX629815) dilution: 1:1000
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATG12 antibody [GT166] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX629815

GTX629815 ICC/IF Image

Orthogonal

ATG12 antibody [GT166] detects ATG12 protein at autophagosome by immunofluorescent analysis.
Samples: HeLa cells mock (left) and treated with 50μM Chloroquine for 24 hr (right) were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATG12 protein stained by ATG12 antibody [GT166] (GTX629815) diluted at 1:1000.
Red: Phalloidin, a F-actin marker.

文献引用

Application Reference

Sara Monaci et al. Front Immunol 2020; 11 : 573646 Hypoxia Shapes Autophagy in LPS-Activated Dendritic Cells.
实验应用 : ICC/IF
种属反应 : Human
Lin TY et al. Autophagy 2019; : 1-18 BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.
实验应用 : ICC/IF, PLA
种属反应 : Human
Samuel J Kenny et al. bioRxiv 2019; (Epub) Super-resolution microscopy unveils FIP200-scaffolded, cup-shaped organization of mammalian autophagic initiation machinery.
实验应用 : ICC/IF
种属反应 : Human
Chang HP et al. Int J Oncol 2018; (Epub) pterostilbene modulates the suppression of multidrug resistance protein-1 and triggers autophagic and apoptotic mechanisms in *-resistant human oral cancer car cells via akt signaling.
实验应用 : WB
种属反应 : Human
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PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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