ATG12抗体

Name: ATG12抗体
Brand: GeneTex
SKU: GTX124181
Price: 4000.00 RMB

Anti-ATG12 antibody used in Western Blot (WB). GTX124181

Anti-ATG12 antibody used in Immunoprecipitation (IP). GTX124181

Anti-ATG12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124181

目录号 GTX124181

文献引用 14

宿主	Rabbit
克隆	Polyclonal
同种型	IgG
实验应用	WB, ICC/IF, IHC-P, IP
种属反应	Human, Mouse, Rat
产品包装	100 μl (¥4000), 25 μl (¥1700)

查看所有 ATG12 的产品

View product citations for antibody GTX124181 on CiteAb

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认

实验应用	稀释倍数
WB	1:500-1:3000
ICC/IF	Assay dependent
IHC-P	1:100-1:1000
IP	1:100-1:500

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

15 kDa. ( Note )

阳性对照

NIH-3T3 , Rat brain , 293T

预测种属反应

Pig, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.43 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human ATG12. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11171947

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

别名

autophagy related 12 , APG12 , APG12L , FBR93 , HAPG12

细胞定位

Cytoplasm

功能与背景

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. ATG12 is the human homolog of a yeast protein involved in autophagy (Mizushima et al., 1998 [PubMed 9852036]).[supplied by OMIM]

数据库

Gene ID: 9140 ATG12
UniProt: O94817 ATG12

研究领域

產品图片

GTX124181 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181 WB Image

Sample (30 μg of whole cell lysate)
A: NIH-3T3
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181 IP Image

Immunoprecipitation of Atg 12-Atg5 protein from 293T whole cell extracts using 5 μg of ATG12 antibody (GTX124181).
Western blot analysis was performed using ATG12 antibody (GTX124181) diluted at 1:1000.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

GTX124181 IHC-P Image

ATG12 antibody detects ATG12 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
ATG12 antibody (GTX124181) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124181 IHC-P Image

ATG12 antibody detects ATG12 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
ATG12 stained by ATG12 antibody (GTX124181) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX124181 WB Image

Sample (50 μg of whole cell lysate)
A: Rat brain
12% SDS PAGE
GTX124181 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with ATG12 antibody (GTX124181) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124181 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28195146

GTX124181 WB Image

The data was published in the journal Sci Rep in 2017. PMID: 28134285

GTX124181 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28195146

GTX124181 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28195146

GTX124181 WB Image

The data was published in the journal Biomolecules in 2019.PMID: 31505885

文献引用

Application Reference

Kuo-Hao Ho et al. Neurotherapeutics 2021; 18 (2) : 1371-1392 Guanabenz Sensitizes Glioblastoma Cells to Sunitinib by Inhibiting GADD34-Mediated Autophagic Signaling.

实验应用 : WB

种属反应 : Human

Tomoko Okada et al. Int J Mol Sci 2021; 22 (4) Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells.

实验应用 : ICC/IF

种属反应 : Rat

Chen JC et al. Biomolecules 2019; 9 (9) In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells.

实验应用 : WB

种属反应 : Human

Lin TY et al. Autophagy 2019; : 1-18 BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.

实验应用 : WB, IP

种属反应 : Human

Tian Zhong et al. Int J Clin Exp Med 2019; 12 (9) : 11185-11198 microRNA-146a relieves smoking relative chronic obstructive pulmonary disease through targeting ATG12.

实验应用 : IHC-P

种属反应 : Mouse

显示更多 +

我要回报文献

产品评论

目前没有使用 ATG12 antibody (GTX124181) 的产品评论被收录。快发表第一篇产品评论吧！

我要评论产品

Application Reference

Kuo-Hao Ho et al. Neurotherapeutics 2021; 18 (2):1371-1392 Guanabenz Sensitizes Glioblastoma Cells to Sunitinib by Inhibiting GADD34-Mediated Autophagic Signaling.

实验应用 : WB

种属反应 : Human

Tomoko Okada et al. Int J Mol Sci 2021; 22 (4) Scanning Electron-Assisted Dielectric Microscopy Reveals Autophagosome Formation by LC3 and ATG12 in Cultured Mammalian Cells.

实验应用 : ICC/IF

种属反应 : Rat

Chen JC et al. Biomolecules 2019; 9 (9) In Vitro and In Silico Mechanistic Insights into miR-21-5p-Mediated Topoisomerase Drug Resistance in Human Colorectal Cancer Cells.

实验应用 : WB

种属反应 : Human

Lin TY et al. Autophagy 2019;:1-18 BIRC5/Survivin is a novel ATG12-ATG5 conjugate interactor and an autophagy-induced DNA damage suppressor in human cancer and mouse embryonic fibroblast cells.

实验应用 : WB, IP

种属反应 : Human

Tian Zhong et al. Int J Clin Exp Med 2019; 12 (9):11185-11198 microRNA-146a relieves smoking relative chronic obstructive pulmonary disease through targeting ATG12.

实验应用 : IHC-P

种属反应 : Mouse

Hsu HL et al. Int J Mol Sci 2019; 20 (1) Chloramphenicol Induces Autophagy and Inhibits the Hypoxia Inducible Factor-1 Alpha Pathway in Non-Small Cell Lung Cancer Cells.

实验应用 : WB

种属反应 : Human

Liu YH et al. Phytomedicine 2018; 46:184-192 Aqueous extracts of Paeonia suffruticosa modulates mitochondrial proteostasis by reactive oxygen species-induced endoplasmic reticulum stress in pancreatic cancer cells.

实验应用 : WB

种属反应 : Human

Lai CH et al. Cell Microbiol 2018;:e12947 Helicobacter pylori cholesterol glucosylation modulates autophagy for increasing intracellular survival in macrophages.

实验应用 : WB

种属反应 : Mouse

Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.

实验应用 : WB

种属反应 : Mouse

Kuo SM et al. MBio 2017; 8 (3) Inhibition of Avian Influenza A Virus Replication in Human Cells by Host Restriction Factor TUFM Is Correlated with Autophagy.

实验应用 : WB

种属反应 : Human

Yen JH et al. Int J Mol Sci 2017; 18 (4) Fisetin Protects PC12 Cells from Tunicamycin-Mediated Cell Death via Reactive Oxygen Species Scavenging and Modulation of Nrf2-Driven Gene Expression, SIRT1 and MAPK Signaling in PC12 Cells.

实验应用 : WB

种属反应 : Rat

Tsai CH et al. Sci Rep 2017; 7:41927 The inhibition of lung cancer cell migration by AhR-regulated autophagy.

实验应用 : WB

种属反应 : Human

Liu YH et al. Sci Rep 2017; 7:41437 Aqueous extract of Polygonum bistorta modulates proteostasis by ROS-induced ER stress in human hepatoma cells.

实验应用 : WB

种属反应 : Human

Wei RJ et al. Toxicol Appl Pharmacol 2016; (Epub) A microtubule inhibitor, ABT-751, induces autophagy and delays apoptosis in Huh-7 cells.

实验应用 : WB

种属反应 : Human

SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

产品包装	报价 (¥)
100 μl	¥ 4000
25 μl	¥ 1700
Qty

	(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
	http://www.neobioscience.com
	genetex@neobioscience.com

ATG12抗体

目录号 GTX124181

宿主

克隆

同种型

实验应用

种属反应

产品应用

应用说明

预期分子量

阳性对照

预测种属反应

产品属性

形式

存储溶液

存放说明

浓度

免疫原种属

免疫原

纯化方式

偶联

RRID

注意事项

相关讯息

别名

细胞定位

功能与背景

数据库

研究领域

產品图片

GTX124181 WB Image

GTX124181 WB Image

GTX124181 IP Image

GTX124181 IHC-P Image

GTX124181 IHC-P Image

GTX124181 WB Image

GTX124181 WB Image

GTX124181 WB Image

GTX124181 WB Image

GTX124181 WB Image

GTX124181 WB Image

GTX124181 WB Image

文献引用

Application Reference

产品评论

产品

研究

支持

公司

Follow Us

粤ICP备08007346号-9

© 1998-2022 GeneTex, Inc. 保留所有权利。

所有产品仅供研究使用 - 不应用于诊断或治疗。