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ATM抗体 [2C1-RB]

Recombinant antibody KO/KD Orthogonal
Anti-ATM antibody [2C1-RB] used in Western Blot (WB). GTX635395
Anti-ATM antibody [2C1-RB] used in IHC (Paraffin sections) (IHC-P). GTX635395
Anti-ATM antibody [2C1-RB] used in Western Blot (WB). GTX635395
Anti-ATM antibody [2C1-RB] used in Western Blot (WB). GTX635395

目录号 GTX635395

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 2C1-RB

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:1000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

351 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888523

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

细胞定位

Nucleus,Cytoplasmic vesicle

功能与背景

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

数据库

研究领域

產品图片

Anti-ATM antibody [2C1-RB] used in Western Blot (WB). GTX635395

GTX635395 WB Image

KO/KD

Non-transfected (–) and transfected (+) HeLa whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (GTX635395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATM antibody [2C1-RB] used in IHC (Paraffin sections) (IHC-P). GTX635395

GTX635395 IHC-P Image

ATM antibody [2C1-RB] detects ATM protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
ATM stained by ATM antibody [2C1-RB] (GTX635395) diluted at 1:200.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-ATM antibody [2C1-RB] used in Western Blot (WB). GTX635395

GTX635395 WB Image

Various tissue extracts (50 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (GTX635395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATM antibody [2C1-RB] used in Western Blot (WB). GTX635395

GTX635395 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [2C1-RB] (GTX635395) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

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SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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