摘要
ATM antibody [2C1] is a mouse monoclonal antibody developed by Dr. Eva Lee’s lab at the University of Texas Health Science Center at San Antonio (PMID: 8969240). It is a well-validated and highly cited reagent to detect ATM protein, which is a nuclear serine/threonine kinase that plays a pivotal role in DNA damage sensing and repair.
应用说明
Recommended Starting Dilutions:
For WB: Use at a dilution of 1:500-1:3000. Predicted 350 kDa.
For IHC-P: Use at 5 μg/mL. Antigen retrieval in Citrate buffer is recommended.
For IP: Use at a concentration of 1-10 μg/ml.
For ICC/IF: Please refer to the publication by Harry Scherthan, et.al., 2000 and Yiyoung Liu, et.al., 2006.
For FACS:Use at an dependent assay. Optimal dilutions/concentrations should be determined by the researcher.
预期分子量
阳性对照
SK-N-SH , HeLa , HeLa nuclear extract
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein expressed in E. coli corresponding to amino acids 2577-3056.
纯化方式
Purified by antigen-affinity chromatography.
From tissue culture supernatant
偶联
Unconjugated
RRID
AB_368161
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
别名
ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1
细胞定位
Nucleus , Cytoplasmic vesicle
功能与背景
The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]
数据库
研究领域