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ATM抗体 [3E8]

Orthogonal
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in Western Blot (WB). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105
Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

目录号 GTX70105

文献引用 5

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 3E8

同种型

 IgG1

实验应用

 WB, IHC-P, IP

种属反应

 Human, Mouse, Rat, Monkey
产品包装
100 μl (¥4300)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1-2 μg/ml
IHC-P Assay dependent
IP 2-10 μg/ml
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

351 kDa. ( Note )

阳性对照

Raji, HeLa, or Akata

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein expressed in E. coli corresponding to amino acids 980-1512.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_368163

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

细胞定位

Nucleus , Cytoplasmic vesicle

功能与背景

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

数据库

研究领域

產品图片

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Human Kidney (formalin-fixed, paraffin-embedded) stained with ATM antibody at 5 μg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Anti-ATM antibody [3E8] used in Western Blot (WB). GTX70105

GTX70105 WB Image

Orthogonal

HeLa whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM antibody [3E8] (GTX70105) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded U373 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

Anti-ATM antibody [3E8] used in IHC (Paraffin sections) (IHC-P). GTX70105

GTX70105 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using ATM(GTX70105) antibody at 1:200 dilution.

文献引用

Application Reference

Fini L et al. Carcinogenesis 2008; 29 (1) : 139-46 Chemopreventive properties of pinoresinol-rich olive oil involve a selective activation of the ATM-p53 cascade in colon cancer cell lines.
实验应用 : WB
种属反应 : Human
Bai L et al. Gastroenterology 2006; 131 (3) : 841-52 Recruitment of ataxia-telangiectasia mutated to the p21(waf1) promoter by ZBP-89 plays a role in mucosal protection.
Shiotani B et al. Mol Cancer Res 2006; 4 (2) : 125-33 Involvement of the ATR- and ATM-dependent checkpoint responses in cell cycle arrest evoked by pierisin-1.
实验应用 : WB
种属反应 : Human

Clone Reference

Post S et al. Proc Natl Acad Sci U S A 2001; 98 (23):13102-7 Phosphorylation of serines 635 and 645 of human Rad17 is cell cycle regulated and is required for G(1)/S checkpoint activation in response to DNA damage.
Chen MJ et al. J Biol Chem 2001; 276 (19):16580-6 ATM-dependent phosphorylation of human Rad9 is required for ionizing radiation-induced checkpoint activation.
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