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ATM (phospho Ser1981)抗体 [HL1062]

Recombinant antibody Orthogonal
Anti-ATM (phospho Ser1981) antibody [HL1062] used in Western Blot (WB). GTX636086
Anti-ATM (phospho Ser1981) antibody [HL1062] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636086
Anti-ATM (phospho Ser1981) antibody [HL1062] used in Western Blot (WB). GTX636086
Anti-ATM (phospho Ser1981) antibody [HL1062] used in IHC (Paraffin sections) (IHC-P). GTX636086

目录号 GTX636086

文献引用 1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1062

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

351 kDa. ( Note )

预测种属反应

Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1981 of human ATM. The exact sequence is proprietary.

纯化方式

Affinity purified by protein A.

偶联

Unconjugated

RRID

AB_2909941

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ATM serine/threonine kinase , AT1 , ATA , ATC , ATD , ATDC , ATE , TEL1 , TELO1

细胞定位

Nucleus,Cytoplasmic vesicle

功能与背景

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

数据库

研究领域

產品图片

Anti-ATM (phospho Ser1981) antibody [HL1062] used in Western Blot (WB). GTX636086

GTX636086 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (GTX636086) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-ATM (phospho Ser1981) antibody [HL1062] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636086

GTX636086 ICC/IF Image

Orthogonal

ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (GTX636086) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-ATM (phospho Ser1981) antibody [HL1062] used in Western Blot (WB). GTX636086

GTX636086 WB Image

Orthogonal

Untreated (–) and treated (+) 293T whole cell extracts (60 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATM (phospho Ser1981) antibody [HL1062] (GTX636086) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-ATM (phospho Ser1981) antibody [HL1062] used in IHC (Paraffin sections) (IHC-P). GTX636086

GTX636086 IHC-P Image

ATM (phospho Ser1981) antibody [HL1062] detects ATM (phospho Ser1981) protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human cervical carcinoma.
ATM (phospho Ser1981) stained by ATM (phospho Ser1981) antibody [HL1062] (GTX636086) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

文献引用

Application Reference

Yang JL et al. In Vivo 2021; 35 (5) : 2687-2696 Ouabain Induces DNA Damage in Human Osteosarcoma U-2 OS Cells and Alters the Expression of DNA Damage and DNA Repair-associated Proteins.
实验应用 : WB
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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