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ATR抗体 [2B5]

Orthogonal
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109
Anti-ATR antibody [2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70109
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109
Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

目录号 GTX70109

文献引用 29

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 2B5

同种型

 IgG1

实验应用

 WB, ICC/IF, IP, Functional Assay

种属反应

 Human, Mouse
产品包装
100 μl (¥4300)
查看所有 ATR 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Functional Assay Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

301 kDa. ( Note )

阳性对照

Raji , Raji nuclear extract

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant fragment, corresponding to amino acids 710-1100 of Human ATR.

纯化方式

Protein G purified

偶联

Unconjugated

RRID

AB_368158

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

ATR serine/threonine kinase , FCTCS , FRP1 , MEC1 , SCKL , SCKL1

细胞定位

Nucleus

功能与背景

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1. [provided by RefSeq, Aug 2017]

数据库

研究领域

產品图片

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Orthogonal

Raji whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody (GTX70109) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATR antibody [2B5] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX70109

GTX70109 ICC/IF Image

Immunofluorescence analysis of HeLa, using ATR(GTX70109) antibody at 1:100 dilution.

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATR antibody [2B5] (GTX70109) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-ATR antibody [2B5] used in Western Blot (WB). GTX70109

GTX70109 WB Image

Detection of ATM (Anti-ATM 2C1, GTX70103) or ATR (Anti-ATR 2B5,GTX70109) by western blot in human Raji whole cell extract.
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

文献引用

Application Reference

Alzhanova D et al. PLoS Pathog 2021; 17 (1) : e1009033 Novel modulators of p53-signaling encoded by unknown genes of emerging viruses.
实验应用 : WB
种属反应 : Human
Sun X et al. Nucleic Acids Res 2020; 48 (16) : 9109-9123 NRF2 preserves genomic integrity by facilitating ATR activation and G2 cell cycle arrest.
Yenerall P et al. Cell Chem Biol 2020; 27 (1) : 105-121.e14 RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
实验应用 : WB
Jingwen Li et al. RSC Adv 2019; 9 (67) : 39082-39089 New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.
实验应用 : WB
种属反应 : Human
Chen X et al. J Cancer Res Clin Oncol 2019; 145 (6) : 1471-1484 IL-6 signaling contributes to radioresistance of prostate cancer through key DNA repair-associated molecules ATM, ATR, and BRCA 1/2.
实验应用 : ICC/IF
种属反应 : Human
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Application Reference
Alzhanova D et al. PLoS Pathog 2021; 17 (1):e1009033 Novel modulators of p53-signaling encoded by unknown genes of emerging viruses.
实验应用 : WB
种属反应 : Human
Sun X et al. Nucleic Acids Res 2020; 48 (16):9109-9123 NRF2 preserves genomic integrity by facilitating ATR activation and G2 cell cycle arrest.
Yenerall P et al. Cell Chem Biol 2020; 27 (1):105-121.e14 RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.
实验应用 : WB
Jingwen Li et al. RSC Adv 2019; 9 (67):39082-39089 New spirobisnaphthalenes from an endolichenic fungus strain CGMCC 3.15192 and their anticancer effects through the P53-P21 pathway.
实验应用 : WB
种属反应 : Human
Chen X et al. J Cancer Res Clin Oncol 2019; 145 (6):1471-1484 IL-6 signaling contributes to radioresistance of prostate cancer through key DNA repair-associated molecules ATM, ATR, and BRCA 1/2.
实验应用 : ICC/IF
种属反应 : Human
Burger K et al. Nucleic Acids Res 2019; (Epub) Tyrosine kinase c-Abl couples RNA polymerase II transcription to DNA double-strand breaks.
Tu HJ et al. Clin Epigenetics 2018; 10 (1):162 The anticancer effects of MPT0G211, a novel HDAC6 inhibitor, combined with chemotherapeutic agents in human acute leukemia cells.
实验应用 : WB
种属反应 : Human
Chen CC et al. Cancers (Basel) 2018; 10 (9) Melatonin Sensitizes Hepatocellular Carcinoma Cells to Chemotherapy Through Long Non-Coding RNA RAD51-AS1-Mediated Suppression of DNA Repair.
实验应用 : WB
种属反应 : Human
Hu L et al. J Immunol 2018; (Epub) TP53 Haploinsufficiency Rescues Emergency Granulopoiesis in FANCC-/- Mice.
种属反应 : Mouse
Furusawa Y et al. Apoptosis 2017; (Epub) Checkpoint kinase 2 is dispensable for regulation of the p53 response but is required for G2/M arrest and cell survival in cells with p53 defects under heat stress.
实验应用 : WB
种属反应 : Human
Glanzer JG et al. Cancer Res 2014; RPA Inhibition increases Replication Stress and Suppresses Tumor Growth.
实验应用 : WB
Gatei M et al. Hum Mol Genet 2014; RAD50 phosphorylation promotes ATR downstream signaling and DNA restart following replication stress.
实验应用 : ICC/IF
种属反应 : Human
Qin B et al. J Biol Chem 2013; 288 (22):16139-44 Ataxia telangiectasia-mutated- and Rad3-related protein regulates the DNA damage-induced G2/M checkpoint through the Aurora A cofactor Bora protein.
实验应用 : WB
种属反应 : Human
Furusawa Y et al. Apoptosis 2012; 17 (1):102-12 Inhibition of checkpoint kinase 1 abrogates G2/M checkpoint activation and promotes apoptosis under heat stress.
实验应用 : WB
种属反应 : Human
Ha K et al. Mol Cancer Ther 2011; 10 (7):1194-206 Hsp90 inhibitor-mediated disruption of chaperone association of ATR with hsp90 sensitizes cancer cells to DNA damage.
实验应用 : ICC/IF
种属反应 : Human
Chen Y et al. Cell Cycle 2011; 10 (4):655-63 Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR.
实验应用 : WB
种属反应 : Human
Zuco V et al. Cancer Lett 2010; 292 (2):186-96 ATM- and ATR-mediated response to DNA damage induced by a novel camptothecin, ST1968.
实验应用 : ICC/IF
种属反应 : Human
Cui B et al. Mol Pharmacol 2009; 75 (6):1356-63 Bifunctional DNA alkylator 1,3-bis(2-chloroethyl)-1-nitrosourea activates the ATR-Chk1 pathway independently of the mismatch repair pathway.
实验应用 : WB
种属反应 : Human
Katsuki Y et al. Cancer Sci 2008; 99 (8):1539-45 Caffeine yields aneuploidy through asymmetrical cell division caused by misalignment of chromosomes.
实验应用 : WB
种属反应 : Human
Kedar PS et al. DNA Repair (Amst) 2008; 7 (11):1787-98 Interaction between PARP-1 and ATR in mouse fibroblasts is blocked by PARP inhibition.
Liu X et al. Mol Cancer Ther 2008; 7 (1):133-42 Ataxia-telangiectasia and Rad3-related and DNA-dependent protein kinase cooperate in G2 checkpoint activation by the DNA strand-breaking nucleoside analogue 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine.
实验应用 : WB
种属反应 : Human
Kulkarni A et al. Am J Physiol Lung Cell Mol Physiol 2008; 294 (5):L998-L1006 Differential roles of ATR and ATM in p53, Chk1, and histone H2AX phosphorylation in response to hyperoxia: ATR-dependent ATM activation.
实验应用 : Functional Assay
种属反应 : Human
Manthey KC et al. J Cell Sci 2007; 120 (Pt 23):847973 NBS1 mediates ATR-dependent RPA hyperphosphorylation following replication-fork stall and collapse.
实验应用 : WB
种属反应 : Human
Wu X et al. Oncogene 2007; 26 (5):757-64 ATR-dependent checkpoint modulates XPA nuclear import in response to UV irradiation.
实验应用 : ICC/IF
种属反应 : Human
Liu Y et al. J Cell Sci 2006; 119 (Pt 22):1002471 DNA damage responses in progeroid syndromes arise from defective maturation of prelamin A.
实验应用 : ICC/IF
种属反应 : Human
Wu X et al. Cancer Res 2006; 66 (6):2997-3005 Phosphorylation of nucleotide excision repair factor xeroderma pigmentosum group A by ataxia telangiectasia mutated and Rad3-related-dependent checkpoint pathway promotes cell survival in response to UV irradiation.
实验应用 : ICC/IF
种属反应 : Human
Horton JK et al. J Biol Chem 2005; 280 (16):15773-85 Poly(ADP-ribose) polymerase activity prevents signaling pathways for cell cycle arrest after DNA methylating agent exposure.
实验应用 : WB
Li J et al. J Biol Chem 2005; 280 (12):12041-50 Regulation of CHK2 by DNA-dependent protein kinase.
实验应用 : WB
种属反应 : Human
Gibson SL et al. Cancer Res 2005; 65 (23):10734-41 Hypoxia-induced phosphorylation of Chk2 in an ataxia telangiectasia mutated-dependent manner.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4300
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