产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Mouse, Rat, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1.88 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the C-terminus region of human ATRX. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
RRID
AB_2888535
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ATRX chromatin remodeler , JMS , MRX52 , RAD54 , RAD54L , XH2 , XNP , ZNF-HX
细胞定位
Nucleus
功能与背景
The protein encoded by this gene contains an ATPase/helicase domain, and thus it belongs to the SWI/SNF family of chromatin remodeling proteins. This protein is found to undergo cell cycle-dependent phosphorylation, which regulates its nuclear matrix and chromatin association, and suggests its involvement in the gene regulation at interphase and chromosomal segregation in mitosis. Mutations in this gene are associated with X-linked syndromes exhibiting cognitive disabilities as well as alpha-thalassemia (ATRX) syndrome. These mutations have been shown to cause diverse changes in the pattern of DNA methylation, which may provide a link between chromatin remodeling, DNA methylation, and gene expression in developmental processes. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2017]
数据库
研究领域
產品图片
![Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478](/templates/default/js/lightbox/images/loading.gif "Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478")
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GTX635478 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL1210] (GTX635478) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX635478 ICC/IF Image
ATRX antibody [HL1210] detects ATRX protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ATRX stained by ATRX antibody [HL1210] (GTX635478) diluted at 1:100.Red: phalloidin, a cytoskeleton marker, diluted at 1:200.Scale bar= 10 μm.
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GTX635478 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with ATRX antibody [HL1210] (GTX635478) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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文献引用
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产品评论
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![Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478](/upload/website/prouct_img/small/GTX635478/GTX635478_43964_20230728_WB_shRNA_watermark_23073119_192.webp "Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478")
![Anti-ATRX antibody [HL1210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635478](/upload/website/prouct_img/small/GTX635478/GTX635478_43992_20200715_ICC_IF_w_23061202_545.webp "Anti-ATRX antibody [HL1210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635478")
![Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478](/upload/website/prouct_img/small/GTX635478/GTX635478_43901_20230707_WB_TPM_watermark_23071223_842.webp "Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478")
![Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478](/upload/website/prouct_img/normal/GTX635478/GTX635478_43964_20230728_WB_shRNA_watermark_23073119_192.webp "Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478")
![Anti-ATRX antibody [HL1210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635478](/upload/website/prouct_img/normal/GTX635478/GTX635478_43992_20200715_ICC_IF_w_23061202_545.webp "Anti-ATRX antibody [HL1210] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635478")
![Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478](/upload/website/prouct_img/normal/GTX635478/GTX635478_43901_20230707_WB_TPM_watermark_23071223_842.webp "Anti-ATRX antibody [HL1210] used in Western Blot (WB). GTX635478")
