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AUF1抗体 [N1C1]

Anti-AUF1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101813
Anti-AUF1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101813
Anti-AUF1 antibody [N1C1] used in Western Blot (WB). GTX101813

目录号 GTX101813

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF

种属反应

Human
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

38 kDa. ( Note )

阳性对照

A431

预测种属反应

Mouse, Rat, Bovine, Xenopus tropicalis, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.69 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human AUF1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11166847

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

heterogeneous nuclear ribonucleoprotein D , AUF1 , AUF1A , HNRPD , P37 , hnRNPD0

细胞定位

Nucleus

功能与背景

This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-AUF1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101813

GTX101813 ICC/IF Image

AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AUF1 protein stained by AUF1 antibody [N1C1] (GTX101813) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [B-5-1-2] (GTX11304) diluted at 1:10000.
Blue: Hoechst 33342 staining.

Anti-AUF1 antibody [N1C1] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101813

GTX101813 ICC/IF Image

AUF1 antibody [N1C1] detects AUF1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: AUF1 protein stained by AUF1 antibody [N1C1] (GTX101813) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

Anti-AUF1 antibody [N1C1] used in Western Blot (WB). GTX101813

GTX101813 WB Image

Sample (30 ug of whole cell lysate)
A: A431
10% SDS PAGE
GTX101813 diluted at 1:1000

文献引用

产品评论

AUF1 antibody [N1C1] 目录号 GTX101813

Rating ( Average 3 based on 1 users reviews)
实验应用
Western Blot(WB) ( Average 3 based on 1 users reviews)
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : WB
Sample Species : Hu
Sample : Panc1 cells
Amount used : 30μg
Blocking : 5% milk, 37°C, 1Hr
Primary Antibody : 1:1000, 25°C, 2Hr
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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