产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500 - 1:2000 |
| 1:50 - 1:100 |
| 1:50 - 1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品属性
形式
Liquid
存储溶液
PBS, 50% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
Batch dependent (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-306 of human HNRNPD (NP_002129.2).
纯化方式
Purified by affinity chromatography
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
heterogeneous nuclear ribonucleoprotein D , AUF1 , AUF1A , HNRPD , P37 , hnRNPD0
细胞定位
Nucleus,Cytoplasm
功能与背景
This gene belongs to the subfamily of ubiquitously expressed heterogeneous nuclear ribonucleoproteins (hnRNPs). The hnRNPs are nucleic acid binding proteins and they complex with heterogeneous nuclear RNA (hnRNA). These proteins are associated with pre-mRNAs in the nucleus and appear to influence pre-mRNA processing and other aspects of mRNA metabolism and transport. While all of the hnRNPs are present in the nucleus, some seem to shuttle between the nucleus and the cytoplasm. The hnRNP proteins have distinct nucleic acid binding properties. The protein encoded by this gene has two repeats of quasi-RRM domains that bind to RNAs. It localizes to both the nucleus and the cytoplasm. This protein is implicated in the regulation of mRNA stability. Alternative splicing of this gene results in four transcript variants. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
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GTX55526 IHC-P Image
IHC-P analysis of rat lung tissue using GTX55526 AUF1 antibody.
Dilution : 1:100
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GTX55526 WB Image
WB analysis of various sample lysates using GTX55526 AUF1 antibody.
Dilution : 1:1000
Loading : 25μg per lane
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GTX55526 ICC/IF Image
ICC/IF analysis of U2OS cells using GTX55526 AUF1 antibody.
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GTX55526 IHC-P Image
IHC-P analysis of mouse pancreas tissue using GTX55526 AUF1 antibody.
Dilution : 1:100
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GTX55526 IHC-P Image
IHC-P analysis of human stomach tissue using GTX55526 AUF1 antibody.
Dilution : 1:100
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