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Acta2抗体

Anti-Acta2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124505
Anti-Acta2 antibody used in Western Blot (WB). GTX124505
Anti-Acta2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124505
Anti-Acta2 antibody used in Western Blot (WB). GTX124505

目录号 GTX124505

文献引用 6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, IHC-Fr, IHC-Wm

种属反应

 Human, Zebrafish
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 zebrafish Acta2 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:5000-1:20000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

阳性对照

zebrafish muscle , whole zebrafish

预测种属反应

Human (100%)| Mouse (100%)| Rat (100%)| Rabbit (100%)| Bovine (100%)| Chicken (100%)| Pig (100%)| Rhesus Monkey (100%)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1.3 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原

The immunogen used to generate this antibody corresponds to zebrafish Acta2

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11166745

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

actin alpha 2, smooth muscle , wu:fb63d03

数据库

研究领域

產品图片

Anti-Acta2 antibody used in IHC (Whole mount) (IHC-Wm). GTX124505

GTX124505 IHC-Wm Image

Acta2 antibody detects Acta2 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 1 day-post-fertilization zebrafish embryo.
Acta2 antibody (GTX124505) dilution: 1:100.
Anti-Acta2 antibody used in Western Blot (WB). GTX124505

GTX124505 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with  alpha Smooth Muscle Actin antibody (GTX124505) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Acta2 antibody used in IHC (Paraffin sections) (IHC-P). GTX124505

GTX124505 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using Acta2 (GTX124505) antibody at 1:300 dilution.

Anti-Acta2 antibody used in Western Blot (WB). GTX124505

GTX124505 WB Image

Whole zebrafish extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Acta2 antibody (GTX124505) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Shin K et al. bioRxiv 2024; Harnessing the regenerative potential of interleukin11 to enhance heart repair.
WA Sebastian et al. J Hum Genet 2024; Cardiac manifestations of human ACTA2 variants recapitulated in a zebrafish model
实验应用 : IHC-Fr
Zada A et al. Hum Mol Genet 2023; 32 (1) : 151-160 The long Filamin-A isoform is required for intestinal development and motility: implications for chronic intestinal pseudo-obstruction.
实验应用 : IHC-P
种属反应 : Zebrafish
Allanki S et al. Sci Adv 2021; 7 (37) : eabg6497 Interleukin-11 signaling promotes cellular reprogramming and limits fibrotic scarring during tissue regeneration.
实验应用 : IHC-Fr
种属反应 : Zebrafish
Chen HH et al. iScience 2021; 24 (9) : 103086 DDX3 modulates the tumor microenvironment via its role in endoplasmic reticulum-associated translation.
实验应用 : WB
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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