产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| 1:100-1:1000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
NIH-3T3 , JC , BCL-1 , A431 , PC-12 , Rat2
预测种属反应
Bovine, Chicken, Xenopus tropicalis, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 10% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
0.2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the N-terminus region of human Actin Gamma 1. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_2036153
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
actin gamma 1 , ACT , ACTG , DFNA20 , DFNA26 , HEL-176
细胞定位
Cytoplasm , cytoskeleton
功能与背景
Actins are highly conserved proteins that are involved in various types of cell motility, and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in nonmuscle cells. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX101794 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Sarcomeric Actin antibody (GTX101794) diluted at 1:1000.
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GTX101794 IHC-P Image
Immunohistochemical analysis of paraffin-embedded human Spleen, using gamma Actin(GTX101794) antibody(10 μg/ml).
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX101794 WB Image
Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
10% SDS PAGE
GTX101794 diluted at 1:1000
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GTX101794 WB Image
Sample (30 ug of whole cell lysate)
A: NIH-3T3
B: JC
C: BCL-1
10% SDS PAGE
GTX101794 diluted at 1:1000
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GTX101794 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin Gamma 1 antibody (GTX101794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX101794 WB Image
PG-4 whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Actin Gamma 1 antibody (GTX101794) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX101794 IHC-P Image
Actin Gamma 1 antibody detects Actin Gamma 1 protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded cat intestine.Actin Gamma 1 stained by Actin Gamma 1 antibody (GTX101794) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX101794 WB Image
The data was published in the journal J Diabetes Res in 2018.PMID: 29955616
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GTX101794 WB Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31430901
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GTX101794 WB Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31430901
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GTX101794 WB Image
The data was published in the journal Cancers (Basel) in 2019.PMID: 31430901
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文献引用
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