产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody was raised against human Adenosine A1 Receptor Intracellular domain.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Synthetic peptide encompassing a sequence within the Intracellular domain of human Adenosine A1 Receptor. The exact sequence is proprietary.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
adenosine A1 receptor , RDC7
细胞定位
Cell membrane
功能与背景
The protein encoded by this gene is an adenosine receptor that belongs to the G-protein coupled receptor 1 family. There are 3 types of adenosine receptors, each with a specific pattern of ligand binding and tissue distribution, and together they regulate a diverse set of physiologic functions. The type A1 receptors inhibit adenylyl cyclase, and play a role in the fertilization process. Animal studies also suggest a role for A1 receptors in kidney function and ethanol intoxication. Transcript variants with alternative splicing in the 5' UTR have been found for this gene. [provided by RefSeq, Jul 2008]
数据库
研究领域
產品图片
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/templates/default/js/lightbox/images/loading.gif "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
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GTX638758 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638758 WB Image
Non-transfected (–) and transfected (+) MCF-7 whole cell extract (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638758 WB Image
Non-transfected (–) and transfected (+) MCF-7 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000 and competitor's antibody (competitor) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX638758 WB Image
Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membranes were blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:2000 and the highly cited competitor's diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
*The competitor is not affiliated with GeneTex and does not endorse this product.
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GTX638758 IHC-P Image
Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat cerebellum.Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638758 WB Image
Whole cell extract (30 μg) was separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX638758 WB Image
SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX638758 IHC-P Image
Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638758 IHC-P Image
Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded rat tissue.Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638758 IHC-P Image
Adenosine A1 Receptor antibody [HL2442] detects Adenosine A1 Receptor protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse cerebellum.Adenosine A1 Receptor stained by Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:200.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX638758 WB Image
Boiled and unboiled SH-SY5Y whole cell and membrane extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Adenosine A1 Receptor antibody [HL2442] (GTX638758) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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文献引用
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产品评论
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![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_T-45075_20230811_WB_shRNA_watermark_23081619_744.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_T-45075_20230707_WB_shRNA_watermark_23071223_401.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_45159_20240223_WB_shRNA_competitor_watermark_24022619_670.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_45159_20240301_WB_Fraction_competitor_watermark_24030600_600.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_T-45075_20230714_IHC-P_R_23081619_861.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_45159_20230908_WB_D_23091319_905.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_45159_20230922_WB_Fraction_23100118_931.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_45159_20230928_IHC-P_M_23100319_756.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_45159_20230928_IHC-P_multiple_R_23100319_737.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_45159_20231020_IHC-P_M_23102401_738.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/small/GTX638758/GTX638758_T-45075_20230707_WB_Fraction_23071223_875.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_T-45075_20230811_WB_shRNA_watermark_23081619_744.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_T-45075_20230707_WB_shRNA_watermark_23071223_401.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20240223_WB_shRNA_competitor_watermark_24022619_670.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20240301_WB_Fraction_competitor_watermark_24030600_600.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_T-45075_20230714_IHC-P_R_23081619_861.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20230908_WB_D_23091319_905.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20230922_WB_Fraction_23100118_931.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20230928_IHC-P_M_23100319_756.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20230928_IHC-P_multiple_R_23100319_737.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_45159_20231020_IHC-P_M_23102401_738.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in IHC (Paraffin sections) (IHC-P). GTX638758")
![Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758](/upload/website/prouct_img/normal/GTX638758/GTX638758_T-45075_20230707_WB_Fraction_23071223_875.webp "Anti-Adenosine A1 Receptor antibody [HL2442] used in Western Blot (WB). GTX638758")
