Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Adiponectin antibody detects Adiponectin protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Adiponectin antibody detects Adiponectin protein at cytoplasm and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Adiponectin stained by Adiponectin antibody (GTX112777) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.