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Adiponectin抗体

Orthogonal
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in IHC (Paraffin sections) (IHC-P). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

目录号 GTX112777

文献引用 20
4

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P

种属反应

 Human, Mouse, Rat, Plant
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

26 kDa. ( Note )

阳性对照

Human plasma , Mouse plasma , rat plasma

预测种属反应

Rabbit, Sheep, Bovine, Cat, Dog, Pig, Rhesus Monkey(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Adiponectin Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2885323

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

adiponectin, C1Q and collagen domain containing , ACDC , ACRP30 , ADIPQTL1 , ADPN , APM-1 , APM1 , GBP28

细胞定位

Secreted

功能与背景

This gene is expressed in adipose tissue exclusively. It encodes a protein with similarity to collagens X and VIII and complement factor C1q. The encoded protein circulates in the plasma and is involved with metabolic and hormonal processes. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Mouse tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Anti-Adiponectin antibody used in IHC (Paraffin sections) (IHC-P). GTX112777

GTX112777 IHC-P Image

Adiponectin antibody detects Adiponectin protein at cytoplasm and secreted by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brown adipocyte.
Adiponectin stained by Adiponectin antibody (GTX112777) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Adiponectin antibody detects Adiponectin protein by western blot analysis. Human tissue extracts (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500.

Anti-Adiponectin antibody used in Western Blot (WB). GTX112777

GTX112777 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Adiponectin antibody (GTX112777) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Felix JB et al. Res Sq 2024; N-acetylaspartate from fat cells regulates postprandial body temperature.
Abgrall A et al. Cells 2022; 11 (17) Evidence for the Neuronal Expression and Secretion of Adiponectin.
Wen S et al. Int J Mol Sci 2022; 23 (19) Contraction Band Necrosis with Dephosphorylated Connexin 43 in Rat Myocardium after Daily Cocaine Administration.
实验应用 : WB
种属反应 : Rat
Tews D et al. Int J Obes (Lond) 2022; 46 (11) : 1939-1947 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biology.
实验应用 : WB
种属反应 : Human
Cox AR et al. Cell Metab 2022; 34 (12) : 1932-1946.e7 The rheumatoid arthritis drug auranofin lowers leptin levels and exerts antidiabetic effects in obese mice.
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产品评论

Adiponectin antibody 目录号 GTX112777

Rating
( Average 4.5 based on 4 users reviews)
实验应用
Western Blot(WB)
( Average 4.5 based on 4 users reviews)
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Date : Anonymous submitted on 27-Dec-2020
Score :
Application Tested : WB
Sample Species : Rat
Sample : Rat plasma
Amount used : 5 μg
Blocking : 2% skimmed milk, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Date : Anonymous submitted on 31-May-2018
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 50 μg
Primary Antibody : 1:1000, 4°C, 16Hr
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Date : Anonymous submitted on 23-Dec-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : white preadipocytes cells
Amount used : 10μg
Blocking : 5% w/v nonfat milk in TBST
Primary Antibody : 1:1000, 25°C, 2Hr
Anti-Adiponectin antibody used in Western Blot (WB). GTX112777
Date : Anonymous submitted on 26-Aug-2013
Score :
Application Tested : WB
Sample Species : Ms
Sample : 3T3-L1 cells
Amount used : 120μg
Blocking : 5% skim milk, 25°C, 2Hr
Primary Antibody : 1:1000, 25°C, 2Hr
我要评论产品
Application Reference
Felix JB et al. Res Sq 2024; N-acetylaspartate from fat cells regulates postprandial body temperature.
Abgrall A et al. Cells 2022; 11 (17) Evidence for the Neuronal Expression and Secretion of Adiponectin.
Wen S et al. Int J Mol Sci 2022; 23 (19) Contraction Band Necrosis with Dephosphorylated Connexin 43 in Rat Myocardium after Daily Cocaine Administration.
实验应用 : WB
种属反应 : Rat
Tews D et al. Int J Obes (Lond) 2022; 46 (11):1939-1947 20 Years with SGBS cells - a versatile in vitro model of human adipocyte biology.
实验应用 : WB
种属反应 : Human
Cox AR et al. Cell Metab 2022; 34 (12):1932-1946.e7 The rheumatoid arthritis drug auranofin lowers leptin levels and exerts antidiabetic effects in obese mice.
Saveta G Mladenova et al. Front Pharmacol 2021; 12:707507 Anti-Adipogenic Effect of Alchemilla monticola is Mediated Via PI3K/AKT Signaling Inhibition in Human Adipocytes.
实验应用 : WB
种属反应 : Human
Wu H et al. Cells 2021; 10 (11) microRNA-27a-3p but Not -5p Is a Crucial Mediator of Human Adipogenesis.
实验应用 : WB
种属反应 : Human
Savova MS et al. Biomed Pharmacother 2021; 141:111934 Ziziphus jujuba Mill. leaf extract restrains adipogenesis by targeting PI3K/AKT signaling pathway.
实验应用 : WB
种属反应 : Plant
Cox AR et al. Mol Metab 2021; 48:101221 Ube2i deletion in adipocytes causes lipoatrophy in mice.
实验应用 : WB
Vasileva LV et al. Food Chem Toxicol 2021; 149:112002 Rosmarinic acid attenuates obesity and obesity-related inflammation in human adipocytes.
实验应用 : WB
种属反应 : Human
Liliya V Vasileva et al. Int J Mol Sci 2020; 21 (24) Caffeic and Chlorogenic Acids Synergistically Activate Browning Program in Human Adipocytes: Implications of AMPK- and PPAR-Mediated Pathways.
实验应用 : WB
种属反应 : Human
Roos J et al. Cell Mol Life Sci 2020; (Epub) miR-146a regulates insulin sensitivity via NPR3.
实验应用 : WB
种属反应 : Mouse
Saha PK et al. Am J Physiol Endocrinol Metab 2020; 319 (4):E667-E677 miR-30a targets gene networks that promote browning of human and mouse adipocytes.
实验应用 : WB
种属反应 : Human
Chernis N et al. Am J Physiol Cell Physiol 2019; Bisphenol AF promotes inflammation in human white adipocytes.
实验应用 : WB
种属反应 : Human
Tews D et al. Redox Biol 2019; 26:101286 Elevated UCP1 levels are sufficient to improve glucose uptake in human white adipocytes.
实验应用 : WB
种属反应 : Mouse
Koh EH et al. Diabetes 2018; (Epub) mir-30a remodels subcutaneous adipose tissue inflammation to improve * sensitivity in obesity.
实验应用 : WB
种属反应 : Mouse
Chou CL et al. PLoS One 2017; 12 (7):e0180712 Renin inhibition improves metabolic syndrome, and reduces angiotensin II levels and oxidative stress in visceral fat tissues in fructose-fed rats.
实验应用 : WB
种属反应 : Rat
Schendel SA et al. Aesthet Surg J 2017; (Epub) Autologous Adipose-Derived Tissue Matrix Part I: Biologic Characteristics.
实验应用 : WB
种属反应 : Human
Tsai JR et al. PLoS One 2015; 10 (12):e0144462 Curcumin Inhibits Non-Small Cell Lung Cancer Cells Metastasis through the Adiponectin/NF-κb/MMPs Signaling Pathway.
实验应用 : WB, IHC-P
种属反应 : Human
Hartig SM et al. Mol Endocrinol 2015; 29 (9):1320-33 Ubc9 Impairs Activation of the Brown Fat Energy Metabolism Program in Human White Adipocytes.
实验应用 : WB
种属反应 : Human
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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