产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human ALPL protein, and it does not cross react with human ALPI, ALPP, and ALPG protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of Alkaline Phosphatase (Tissue Non-Specific).
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
alkaline phosphatase, biomineralization associated , AP-TNAP , APTNAP , HOPS , TNALP , TNAP , TNSALP
细胞定位
Cell membrane
功能与背景
This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]
数据库
研究领域
產品图片
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/templates/default/js/lightbox/images/loading.gif "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
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GTX637904 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637904 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637904 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637904 WB Image
Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] (GTX637904) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/small/GTX637904/GTX637904_45012_20230804_WB_23080901_450.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/small/GTX637904/GTX637904_T-44872_20230303_WB_multiple_B_23030717_526.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/small/GTX637904/GTX637904_45012_20230505_WB_23050918_741.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/small/GTX637904/GTX637904_45012_20230616_WB_D_23062019_536.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230804_WB_23080901_450.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/normal/GTX637904/GTX637904_T-44872_20230303_WB_multiple_B_23030717_526.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230505_WB_23050918_741.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
![Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904](/upload/website/prouct_img/normal/GTX637904/GTX637904_45012_20230616_WB_D_23062019_536.webp "Anti-Alkaline Phosphatase (Tissue Non-Specific) antibody [HL2003] used in Western Blot (WB). GTX637904")
