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Androgen Receptor (ARv7 Splice Variant)抗体 [HL1028]

Recombinant antibody Comparison Orthogonal
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Western Blot (WB). GTX635842
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Western Blot (WB). GTX635842
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Western Blot (WB). GTX635842
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in IHC (Paraffin sections) (IHC-P). GTX635842
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635842
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Western Blot (WB). GTX635842

目录号 GTX635842

1

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1028

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

99 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Androgen Receptor (ARv7 Splice Variant). The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2909909

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM

细胞定位

Nucleus,Cytoplasm

功能与背景

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

数据库

研究领域

產品图片

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Western Blot (WB). GTX635842

GTX635842 WB Image

Comparison Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membranes were blotted with Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] (GTX635842) and clone [RM7] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Western Blot (WB). GTX635842

GTX635842 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted antibodies as follows:
Upper image : Androgen Receptor (ARv7 Splice Variant) antibody [HL1028], GTX635842 (1:1000)
Lower image : Androgen Receptor antibody [HL1049], GTX636021.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Western Blot (WB). GTX635842

GTX635842 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] (GTX635842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in IHC (Paraffin sections) (IHC-P). GTX635842

GTX635842 IHC-P Image

Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] detects Androgen Receptor (ARv7 Splice Variant) antiboy protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
Androgen Receptor (ARv7 Splice Variant) antiboy stained by Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] (GTX635842) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX635842

GTX635842 ICC/IF Image

Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] detects Androgen Receptor (ARv7 Splice Variant) protein at nucleus by immunofluorescent analysis.
Sample: 22RV1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] (GTX635842) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in Western Blot (WB). GTX635842

GTX635842 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor (ARv7 Splice Variant) antiboy [HL1028] (GTX635842) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品评论

Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] 目录号 GTX635842

Rating
( Average 5 based on 1 users reviews)
实验应用
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1028] used in IHC (Paraffin sections) (IHC-P). GTX635842
Date : Anonymous submitted on 24-May-2022
Score :
Application Tested : IHC-P
Sample Species : Hu
Sample : human metastatic castration-resistant prostate cancer section
Permeabilization /Antigen retrieval : citrate
Primary Antibody : Undiluted, °C, Hr
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产品包装 报价 (¥)
¥ 4000
¥ 1700
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