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Androgen Receptor (ARv7 Splice Variant)抗体 [HL1239]

Recombinant antibody
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636638
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636638
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Western Blot (WB). GTX636638
Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Western Blot (WB). GTX636638

目录号 GTX636638

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 HL1239

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P (cell pellet)

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF Assay dependent
IHC-P (cell pellet) Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

99 kDa. ( Note )

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

1 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Androgen Receptor (ARv7 Splice Variant). The exact sequence is proprietary.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2910020

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

androgen receptor , AIS , AR8 , DHTR , HUMARA , HYSP1 , KD , NR3C4 , SBMA , SMAX1 , TFM

细胞定位

Nucleus,Cytoplasm

功能与背景

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (SBMA, also known as Kennedy's disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2017]

数据库

研究领域

產品图片

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX636638

GTX636638 ICC/IF Image

Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] detects Androgen Receptor (ARv7 Splice Variant) protein by immunofluorescent analysis.
Sample: 22RV1, LNCap and PC-3 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] (GTX636638) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in IHC-P (cell pellet) (IHC-P (cell pellet)). GTX636638

GTX636638 IHC-P (cell pellet) Image

Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] detects Androgen Receptor (ARv7 Splice Variant) protein at nucleus by immunohistochemical analysis.
Sample: PC-3 and 22RV1 FFPE Cell Pellet Block.
Green: Androgen Receptor (ARv7 Splice Variant) stained by Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] (GTX636638) diluted at 1:200.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Western Blot (WB). GTX636638

GTX636638 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] (GTX636638) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Androgen Receptor (ARv7 Splice Variant) antibody [HL1239] used in Western Blot (WB). GTX636638

GTX636638 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted antibodies as follows:
Upper image : Androgen Receptor (ARv7 Splice Variant) antibody [HL1239], GTX636638 (1:1000)
Lower image : Androgen Receptor antibody [HL1049], GTX636021.
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

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产品包装 报价 (¥)
¥ 4000
¥ 1700
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