GeneTex中国官方网站
  • China

Annexin A1抗体

KO/KD Comparison Orthogonal Overexpression
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101070
Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070
Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101070
Anti-Annexin A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101070
Anti-Annexin A1 antibody used in Immunoprecipitation (IP). GTX101070

目录号 GTX101070

文献引用 6

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Annexin A1 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

39 kDa. ( Note )

阳性对照

NIH-3T3 , JC

预测种属反应

Rabbit, Bovine, Dog, Pig, Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.95 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human Annexin A1. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036236

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

annexin A1 , ANX1 , LPC1

细胞定位

Nucleus , Cytoplasm , Cell projection , cilium , Basolateral cell membrane

功能与背景

Annexin I belongs to a family of Ca(2+)-dependent phospholipid binding proteins which have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin I protein has an apparent relative molecular mass of 40 kDa, with phospholipase A2 inhibitory activity. Since phospholipase A2 is required for the biosynthesis of the potent mediators of inflammation, prostaglandins and leukotrienes, annexin I may have potential anti-inflammatory activity. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

KO/KD

Wild-type (WT) and Annexin A1 knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Comparison

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membranes were blotted with Annexin A1 antibody (GTX101070) diluted at 1:5000 and competitor's antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

*The competitor is not affiliated with GeneTex and does not endorse this product.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101070

GTX101070 IHC-P Image

Immunohistochemical analysis of paraffin-embedded N87 Xenograft , using Annexin I(GTX101070) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Annexin A1 antibody used in Western Blot (WB). GTX101070

GTX101070 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin A1 antibody (GTX101070) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Annexin A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX101070

GTX101070 IHC-P Image

Annexin A1 antibody detects Annexin A1 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Annexin A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101070

GTX101070 ICC/IF Image

Annexin A1 antibody detects Annexin A1 protein by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Annexin A1 stained by Annexin A1 antibody (GTX101070) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Annexin A1 antibody used in Immunoprecipitation (IP). GTX101070

GTX101070 IP Image

Immunoprecipitation of Annexin A1 protein from HeLa whole cell extracts using 5 μg of Annexin A1 antibody (GTX101070).
Western blot analysis was performed using Annexin A1 antibody (GTX101070).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

文献引用

Application Reference

Jing Chen et al. Front Immunol 2022; 13 : 875412 HA/CD44 Regulates the T Helper 1 Cells Differentiation by Activating Annexin A1/Akt/mTOR Signaling to Drive the Pathogenesis of EAP.
实验应用 : WB
种属反应 : Mouse
Santarsiero A et al. Oxid Med Cell Longev 2021; 2021 : 5533793 Phenolic Compounds of Red Wine Aglianico del Vulture Modulate the Functional Activity of Macrophages via Inhibition of NF-κB and the Citrate Pathway.
实验应用 : WB
种属反应 : Human
Kuo CH et al. Cell Biochem Funct 2020; (Epub) Biomarker discovery in highly invasive lung cancer cell through proteomics approaches.
实验应用 : WB
种属反应 : Human
Wang C et al. Environ Toxicol Pharmacol 2019; 72 : 103270 Common differentially expressed proteins were found in mouse cleft palate models induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin and retinoic acid.
实验应用 : WB
种属反应 : Mouse
Fan CY et al. Electrophoresis 2013; 34 (24) : 3305-14 Proteomic and redox-proteomic study on the role of glutathione reductase in human lung cancer cells.
实验应用 : WB
种属反应 : Human
显示更多 +
我要回报文献

产品评论

目前没有使用 Annexin A1 antibody (GTX101070) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com