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Annexin V-Biotin Apoptosis 检验试剂组

Annexin V-Biotin Apoptosis Detection Kit. GTX85587
Annexin V-Biotin Apoptosis Detection Kit. GTX85587

目录号 GTX85587

实验应用

 ICC/IF
产品包装
100 assay (¥7900)
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产品说明

摘要

The Annexin V-Biotin Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, most cell types translocate the membrane phospholipid phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can easily bind to Biotin-conjugated Annexin V, a protein that has a strong natural affinity for PS. Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.

‧ Detection method- Flow cytometry (Ex = 488 nm; Em = 530 nm) using and fluorescence microscopy
‧ Sample type- Living cells (suspension and adherant)
‧ Species reactivity- Mammalian
‧ Applications- Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.

Features and Benefits
‧ Simple one step staining procedure in 30 minutes
‧ Fast and convenient
‧ Annexin V-Biotin can be detected in conjunction with conventional dye-staining using any streptavidine- or avidin-dye reagents, such as (strept)avidine-fluorescein, -peroxidase, -alkaline phosphatase (AP), and -β-gal, etc.

Kit Contents:
Annexin V-Biotin
1X Binding Buffer
Propidium Iodide (PI)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
ICC/IF Assay dependent
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

产品属性

存放说明

Store at +4ºC. Product has an expected shelf life of 12 months.

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

研究领域

產品图片

Annexin V-Biotin Apoptosis Detection Kit. GTX85587

GTX85587 ICC/IF Image

Detection of Apoptosis with Annexin V-EGFP. Apoptosis was induced in Jurkat cells by camptothecin (2 mM) for various times as indicated. Cells were collected and incubated with Annexin V-EGFP for 5 minutes according to the kit instructions. Results were analyzed by fluorescence microscopy (top panel) and flow cytometry (bottom panel).

Annexin V-Biotin Apoptosis Detection Kit. GTX85587

GTX85587 Image

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产品包装 报价 (¥)
¥ 7900
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