产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:50000 |
| 1:50-1:200 |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
阳性对照
PC-12 , Rat2 , A431 , HeLa , HepG2 , GL261 , C8D30 , NIH-3T3 , Raw 264.7 , 293T
预测种属反应
Bovine, Dog, Chimpanzee(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS, 20% Glycerol
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant protein encompassing a sequence within the center region of human Annexin V. The exact sequence is proprietary.
纯化方式
Purified by antigen-affinity chromatography.
偶联
Unconjugated
RRID
AB_1949660
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
annexin A5 , ANX5 , ENX2 , HEL-S-7 , PP4 , RPRGL3
功能与背景
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq]
数据库
研究领域
產品图片
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GTX103250 WB Image
Wild-type (WT) and Annexin V knockout (KO) 293T cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody (GTX103250) diluted at 1:50000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103250 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Annexin V antibody (GTX103250) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103250 WB Image
Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
B. 30 μg Rat2 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103250 FACS Image
Annexin V antibody (GTX103250) detects Annexin V protein by flow cytometry analysis.
Sample: HeLa cell.
Black: Unlabelled sample was used as a control.
Red: Annexin V antibody (GTX103250) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.
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GTX103250 WB Image
Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 μg GL261 whole cell lysate/extract
B. 30 μg C8D30 whole cell lysate/extract
C. 30 μg NIH-3T3 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
|
|
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GTX103250 WB Image
Annexin V antibody detects Annexin V protein by western blot analysis.
A. 30 μg A431 whole cell lysate/extract
B. 30 μg HeLa whole cell lysate/extract
C. 30 μg HepG2 whole cell lysate/extract
10% SDS-PAGE
Annexin V antibody (GTX103250) dilution: 1:5000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX103250 WB Image
The data was published in the 2022 in Front Cell Dev Biol. PMID: 35573689
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文献引用
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