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Apolipoprotein A1抗体

Anti-Apolipoprotein A1 antibody used in Western Blot (WB). GTX112692
Anti-Apolipoprotein A1 antibody used in Western Blot (WB). GTX112692
Anti-Apolipoprotein A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112692
Anti-Apolipoprotein A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112692
Anti-Apolipoprotein A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112692
Anti-Apolipoprotein A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112692
Anti-Apolipoprotein A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112692
Anti-Apolipoprotein A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112692
Anti-Apolipoprotein A1 antibody used in Western Blot (WB). GTX112692

目录号 GTX112692

文献引用 7

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

31 kDa. ( Note )

预测种属反应

Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.58 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Apolipoprotein A1 Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_11179007

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

apolipoprotein A1 , apo(a)

细胞定位

Secreted

功能与背景

This gene encodes apolipoprotein A-I, which is the major protein component of high density lipoprotein (HDL) in plasma. The protein promotes cholesterol efflux from tissues to the liver for excretion, and it is a cofactor for lecithin cholesterolacyltransferase (LCAT) which is responsible for the formation of most plasma cholesteryl esters. This gene is closely linked with two other apolipoprotein genes on chromosome 11. Defects in this gene are associated with HDL deficiencies, including Tangier disease, and with systemic non-neuropathic amyloidosis. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Apolipoprotein A1 antibody used in Western Blot (WB). GTX112692

GTX112692 WB Image

Human plasma (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein A1 antibody used in Western Blot (WB). GTX112692

GTX112692 WB Image

Mouse plasma and rat plasma (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Apolipoprotein A1 antibody (GTX112692) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112692

GTX112692 ICC/IF Image

Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at Golgi apparatus by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Anti-Apolipoprotein A1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112692

GTX112692 ICC/IF Image

Apolipoprotein A1 antibody detects Apolipoprotein A1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112692

GTX112692 IHC-P Image

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human colon.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112692

GTX112692 IHC-P Image

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human lung.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112692

GTX112692 IHC-P Image

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:1000.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein A1 antibody used in IHC (Paraffin sections) (IHC-P). GTX112692

GTX112692 IHC-P Image

Apolipoprotein A1 antibody detects secreted Apolipoprotein A1 protein by immunohistochemical analysis.
Sample: Paraffin-embedded human esophagus.
Apolipoprotein A1 stained by Apolipoprotein A1 antibody (GTX112692) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein A1 antibody used in Western Blot (WB). GTX112692

GTX112692 WB Image

The data was published in the 2022 in Biomedicines. PMID: 35203448

文献引用

Application Reference

D Brambilla et al. ChemRxiv 2023; Sequential separation and profiling of extracellular vesicles using antibody-aptamer conjugates
实验应用 : WB
Konaka H et al. EMBO J 2023; 42 (20) : e112573 Secretion of mitochondrial DNA via exosomes promotes inflammation in Beh?et's syndrome.
Nasibeh Karimi et al. J Extracell Vesicles 2022; 11 (5) : e12213 Tetraspanins distinguish separate extracellular vesicle subpopulations in human serum and plasma - Contributions of platelet extracellular vesicles in plasma samples.
实验应用 : WB
Karin Ekstr?m et al. BMC Cancer 2022; 22 (1) : 50 Characterization of surface markers on extracellular vesicles isolated from lymphatic exudate from patients with breast cancer.
实验应用 : WB
种属反应 : Human
Fausto Gueths Gomes et al. Biomedicines 2022; 10 (2) Synergy of Human Platelet-Derived Extracellular Vesicles with Secretome Proteins Promotes Regenerative Functions.
实验应用 : WB
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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