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Apolipoprotein E抗体 [C2C3], C-term

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053
Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

目录号 GTX100053

文献引用 5
1

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, IHC-Fr, IP, ELISA, Sandwich ELISA

种属反应

 Human
产品包装
100 μl (¥4000),
25 μl (¥1700)
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产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:20000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX635889 / GTX635891, Detection : GTX100053
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

36 kDa. ( Note )

阳性对照

Conditional medium from differentiated human primary preadipocyte , HepG2 , human plasma

预测种属反应

Chimpanzee(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.66 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human Apolipoprotein E. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_1949674

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

apolipoprotein E , AD2 , APO-E , ApoE4 , LDLCQ5 , LPG

细胞定位

Secreted

功能与背景

Chylomicron remnants and very low density lipoprotein (VLDL) remnants are rapidly removed from the circulation by receptor-mediated endocytosis in the liver. Apolipoprotein E, a main apoprotein of the chylomicron, binds to a specific receptor on liver cells and peripheral cells. ApoE is essential for the normal catabolism of triglyceride-rich lipoprotein constituents. The APOE gene is mapped to chromosome 19 in a cluster with APOC1 and APOC2. Defects in apolipoprotein E result in familial dysbetalipoproteinemia, or type III hyperlipoproteinemia (HLP III), in which increased plasma cholesterol and triglycerides are the consequence of impaired clearance of chylomicron and VLDL remnants. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Sample (2.5 μg of whole cell lysate)
A: Conditional medium from human primary preadipocyte
B: Conditional medium from differentiated human primary preadipocyte
12% SDS PAGE
GTX100053 diluted at 1:2000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.

Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E antibody [GT1627] (GTX635891) as capture antibody at concentration of 5 μg/mL and Apolipoprotein E antibody [C2C3], C-term (GTX100053) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Human plasma (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX100053

GTX100053 ICC/IF Image

Apolipoprotein E antibody [C2C3], C-term detects Apolipoprotein E protein at cytoplasm by immunofluorescent analysis.
Sample: THP-1 cells were fixed in ice-cold MeOH for 5 min.
Green: Apolipoprotein E protein stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-Apolipoprotein E antibody [C2C3], C-term used in Immunoprecipitation (IP). GTX100053

GTX100053 IP Image

Apolipoprotein E antibody [C2C3], C-term immunoprecipitates Apolipoprotein E protein in IP experiments.
IP Sample: HepG2 whole cell lysate/extract
A : 30 μg whole cell lysate/extract of Apolipoprotein E protein expressing HepG2 cells
B : Control with 3 μg of pre-immune rabbit IgG
C : Immunoprecipitation of Apolipoprotein E by 3 μg of Apolipoprotein E antibody [C2C3], C-term (GTX100053)
10% SDS-PAGE
The immunoprecipitated Apolipoprotein E protein was detected by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:1000.
EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length Apolipoprotein E protein using GTX100053 as capture antibody at concentration of 5 μg/mL and a mouse monoclonal anti-Apolipoprotein E antibody as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX100053

GTX100053 IHC-P Image

Apolipoprotein E antibody [C2C3], C-term detects secreted Apolipoprotein E protein by immunohistochemical analysis.
Sample: Paraffin-embedded human ovarian cancer.
Apolipoprotein E stained by Apolipoprotein E antibody [C2C3], C-term (GTX100053) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Apolipoprotein E antibody [C2C3], C-term used in ELISA (ELISA). GTX100053

GTX100053 ELISA Image

Sandwich ELISA detection of recombinant full-length human apolipoprotein E using Apolipoprotein E antibody [GT27711] (GTX635889) as capture antibody at concentration of 5 μg/mL and Apolipoprotein E antibody [C2C3], C-term (GTX100053) as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053

GTX100053 WB Image

Apolipoprotein E antibody [C2C3], C-term detects APOE protein by western blot analysis.
A. 30 μg HepG2 whole cell lysate/extract
12% SDS-PAGE
Apolipoprotein E antibody [C2C3], C-term (GTX100053) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

文献引用

Application Reference

Adaku N et al. Cancer Res 2023; 83 (18) : 3013-3025 Apolipoprotein E2 Stimulates Protein Synthesis and Promotes Melanoma Progression and Metastasis.
Nilsa La Cunza et al. JCI Insight 2021; 6 (9) Mitochondria-dependent phase separation of disease-relevant proteins drives pathological features of age-related macular degeneration.
实验应用 : IHC-Fr
种属反应 : Human
Lai W et al. Int J Nanomedicine 2020; 15 : 1481-1498 A Protein Corona Adsorbed to a Bacterial Magnetosome Affects Its Cellular Uptake.
实验应用 : WB
Seto S et al. Front Microbiol 2019; 10 : 3081 Proteomic Profiling Reveals the Architecture of Granulomatous Lesions Caused by Tuberculosis and Mycobacterium avium Complex Lung Disease.
实验应用 : IHC-P
种属反应 : Human
Thaker K et al. Neurobiol Dis 2016; 93 : 64-77 Increased expression of ApoE and protection from amyloid-beta toxicity in transmitochondrial cybrids with haplogroup K mtDNA.
实验应用 : ICC/IF
种属反应 : Human
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Apolipoprotein E antibody [C2C3], C-term 目录号 GTX100053

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实验应用
Western Blot(WB)
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Anti-Apolipoprotein E antibody [C2C3], C-term used in Western Blot (WB). GTX100053
Date : Anonymous submitted on 26-Aug-2022
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 and Caco-2 cells
Amount used : 50μg
Blocking : 5% milk, 22°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Document
Unmodified.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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