GeneTex中国官方网站
  • China

Arginase 1抗体 [GT5811]

Overexpression
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218
Anti-Arginase 1 antibody [GT5811] used in IHC (Paraffin sections) (IHC-P). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Flow cytometry (FACS). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218
Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218
Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

目录号 GTX634218

文献引用 2

宿主

 Mouse

克隆

 Monoclonal

克隆编号

 GT5811

同种型

 IgG2a

实验应用

 WB, IHC-P, FACS, ELISA, Sandwich ELISA

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 Arginase 1 的产品
产品说明

摘要

Arginase 1 antibody detects arginase 1, an enzyme with a predicted molecular weight of ~35 kDa. Arginase 1 is cytosolic and expressed primarily in hepatocytes, catalyzing the conversion of L-arginine to urea and L-ornithine. The activity of this enzyme has an immunoregulatory function in M2 macrophages.

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
IHC-P 1:100-1:1000
FACS 1:50-1:200
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634218 / GTX113131 / GTX109242, Detection : GTX109242 / GTX113131 / GTX634218
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

35 kDa. ( Note )

阳性对照

Huh-7 , ARG1-transfected 293T

产品说明

This antibody is specific for human Arginase 1 protein, and it does not cross react with human Arginase 2 protein.

预测种属反应

Rabbit, Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

3.26 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Arginase 1 recombinant protein.

纯化方式

Affinity purified by Protein A.

偶联

Unconjugated

RRID

AB_2888429

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

arginase 1

细胞定位

Cytoplasm

功能与背景

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

GTX634218 WB Image

Rat tissue extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in IHC (Paraffin sections) (IHC-P). GTX634218

GTX634218 IHC-P Image

Arginase 1 antibody [GT5811] detects Arginase 1 protein at cytoplasm in mouse liver by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX113131 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Flow cytometry (FACS). GTX634218

GTX634218 FACS Image

Arginase 1 antibody [GT5811] (GTX634218) detects Arginase 1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody [GT5811] (GTX634218) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

GTX634218 WB Image

Whole cell extract (50 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX113131 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in Western Blot (WB). GTX634218

GTX634218 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [GT5811] (GTX634218) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody [GT5811] used in ELISA (ELISA). GTX634218

GTX634218 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

文献引用

Application Reference

Cheng Zhang et al. J Extracell Vesicles 2022; 11 (4) : e12209 Plasma extracellular vesicle derived protein profile predicting and monitoring immunotherapeutic outcomes of gastric cancer.
Lee JD et al. Arch Toxicol 2020; 94 (3) : 887-909 Integration of transcriptomics, proteomics and metabolomics identifies biomarkers for pulmonary injury by polyhexamethylene guanidine phosphate (PHMG-p), a humidifier disinfectant, in rats.
实验应用 : WB
种属反应 : Human
我要回报文献

产品评论

目前没有使用 Arginase 1 antibody [GT5811] (GTX634218) 的产品评论被收录。快发表第一篇产品评论吧！
我要评论产品
SDS
PBS.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
客制化偶联服务
联系经销商
欣博盛生物科技有限公司
欣博盛生物科技有限公司
(全国热线) 4006-800-892 / (深圳) 0755-26755892 / (北京) 010-88594029 / (上海) 021-34613729 / (广州) 18024516375 / (香港) +852-69410778
http://www.neobioscience.com
genetex@neobioscience.com