产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
实验应用 |
稀释倍数 |
1:500-1:3000 |
Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
产品说明
This antibody is specific for human Arginase 1 protein, and it does not cross react with human Arginase 2 protein.
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
2 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragmemt of human Arginase 1
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
arginase 1
细胞定位
Cytoplasm
功能与背景
Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]
数据库
研究领域
產品图片
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GTX637640 WB Image
Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX637640 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:2500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637640 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637640 WB Image
Rat tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:40000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637640 WB Image
Human tissue extract (5 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:20000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637640 IHC-P Image
Arginase 1 antibody [HL1891] detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat liver.Arginase 1 stained by Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637640 IHC-P Image
Arginase 1 antibody [HL1891] detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Arginase 1 stained by Arginase 1 antibody [HL1891] (GTX637640) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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