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Arginase 1抗体

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242
Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242
Anti-Arginase 1 antibody used in Immunoprecipitation (IP). GTX109242
Anti-Arginase 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109242
Anti-Arginase 1 antibody used in Flow cytometry (FACS). GTX109242
Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242
Anti-Arginase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242
Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

目录号 GTX109242

宿主

Rabbit

克隆

Polyclonal

同种型

IgG

实验应用

WB, ICC/IF, IHC-P, IHC-Fr, FACS, IP, ELISA, Sandwich ELISA

种属反应

Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
FACS 1:50-1:200
IP 1:100-1:1000
ELISA 1:1000-1:10000
Sandwich ELISA Assay dependent

Note :

Sandwich ELISA
Capture : GTX634218 / GTX109242, Detection : GTX109242 / GTX634218

WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

35 kDa. ( Note )

阳性对照

HepG2

预测种属反应

Rabbit, Bovine, Pig(>80% identity)

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.26 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Full length human Arginase 1 Recombinant protein.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036264

注意事项

仅供实验室使用。不适用于人类或动物的任何临床,治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

arginase 1

细胞定位

Cytoplasm

功能与背景

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Non-transfected (–) and transfected (+) HepG2 whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242

GTX109242 ELISA Image

Sandwich ELISA detection of non-transfected and transfected 293T whole cell extracts using GTX634218 as capture antibody at concentration of 5 μg/mL and GTX109242 as detection antibody at concentration of 1 μg/mL. Rabbit IgG antibody (HRP) (GTX213110-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242

GTX109242 IHC-P Image

Arginase 1 antibody detects Arginase 1 protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse liver.
Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Arginase 1 antibody used in Immunoprecipitation (IP). GTX109242

GTX109242 IP Image

Immunoprecipitation of Arginase 1 protein from HepG2 whole cell extracts using 5 μg of Arginase 1 antibody (GTX109242).
Western blot analysis was performed using Arginase 1 antibody (GTX109242) diluted at 1:600.
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-Arginase 1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX109242

GTX109242 IHC-Fr Image

Arginase 1 antibody detects Arginase 1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse hippocampus.
Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Arginase 1 antibody used in Flow cytometry (FACS). GTX109242

GTX109242 FACS Image

Arginase 1 antibody (GTX109242) detects ARG1 protein by flow cytometry analysis.
Sample: HepG2 cell.
Black: Unlabelled sample was used as a control.
Red: Arginase 1 antibody (GTX109242) dilution: 1:50.
Acquisition of 20,000 events were collected for FACS analysis.

Anti-Arginase 1 antibody used in ELISA (ELISA). GTX109242

GTX109242 ELISA Image

Sandwich ELISA detection of recombinant full-length Arginase 1 protein using GTX109242 as capture antibody at concentration of 5 μg/mL and GTX634218 as detection antibody at concentration of 1 μg/mL. Mouse IgG antibody (HRP) (GTX213111-01) was diluted at 1:10000 and used to detect the primary antibody.

Anti-Arginase 1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109242

GTX109242 ICC/IF Image

Arginase 1 antibody detects Arginase 1 protein at cytoplasm by immunofluorescent analysis.
Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Arginase 1 stained by Arginase 1 antibody (GTX109242) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10 μm.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Arginase 1 antibody (GTX109242) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal J Neuroinflammation in 2017.PMID: 28196545

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal PLoS One in 2014.PMID: 24658465

Anti-Arginase 1 antibody used in IHC (Paraffin sections) (IHC-P). GTX109242

GTX109242 IHC-P Image

The data was published in the journal Oncotarget in 2016. PMID: 27637084

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal Sci Rep in 2017.PMID: 28211494

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal BMC Microbiol in 2017. PMID: 28835201

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal Physiol Rep in 2013. PMID: 24303100

Anti-Arginase 1 antibody used in Western Blot (WB). GTX109242

GTX109242 WB Image

The data was published in the journal Oncotarget in 2016. PMID: 27637084

文献引用

产品评论

Arginase 1 antibody 目录号 GTX109242

Rating ( Average 4 based on 2 users reviews)
实验应用
Western Blot(WB) ( Average 4 based on 2 users reviews)
Date : Anonymous submitted on 14-Jul-2021
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW264.7 cells
Amount used : 10 μg
Blocking : 5% non-fat milk in TBST, 25°C, 1Hr
Primary Antibody : 1:6000, 4°C, 16Hr
Date : Anonymous submitted on 25-Nov-2015
Score :
Application Tested : WB
Sample Species : Ms
Sample : RAW 264.7 cells
Amount used : 50μg
Blocking : 5% BSA, 26°C, 2Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Application Reference
Panebianco C et al. Cell Death Discov 2023; 9 (1):116
实验应用 : IHC-P
种属反应 : Mouse
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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