产品应用
应用说明
*最佳稀释倍数与浓度应由研究人员确认
| 实验应用 |
稀释倍数 |
| 1:500-1:3000 |
| Assay dependent |
| Assay dependent |
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片
预期分子量
预测种属反应
Zebrafish, Sheep, Bovine, Chicken, Pig, Xenopus tropicalis(>80% identity)
产品属性
形式
Liquid
存储溶液
PBS
存放说明
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
浓度
1 mg/ml (Please refer to the vial label for the specific concentration.)
免疫原种属
Human
免疫原
Recombinant fragment of human Ataxin 3.
纯化方式
Affinity purified by Protein A.
偶联
Unconjugated
注意事项
仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。
相关讯息
别名
ataxin 3 , AT3 , ATX3 , JOS , MJD , MJD1 , SCA3
功能与背景
Machado-Joseph disease, also known as spinocerebellar ataxia-3, is an autosomal dominant neurologic disorder. The protein encoded by this gene contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 12-44 to 52-86 is one cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2016]
数据库
研究领域
產品图片
![Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658](/templates/default/js/lightbox/images/loading.gif "Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658")
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GTX637658 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637658 IHC-P Image
Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded human lung cancer.Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637658 IHC-P Image
Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded rat brain.Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637658 IHC-P Image
Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.Sample: Paraffin-embedded mouse stomach.Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX637658 WB Image
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637658 ICC/IF Image
Ataxin 3 antibody [HL1909] detects Ataxin 3 protein by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Fluoroshield with DAPI (GTX30920).Scale bar= 10μm.
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GTX637658 WB Image
Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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文献引用
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产品评论
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![Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658](/upload/website/prouct_img/small/GTX637658/GTX637658_44900_20230210_WB_shRNA_watermark_23021401_927.webp "Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658](/upload/website/prouct_img/small/GTX637658/GTX637658_T-44837_20221223_IHC-P_22122722_218.webp "Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658](/upload/website/prouct_img/small/GTX637658/GTX637658_T-44837_20221223_IHC-P_R_22122722_521.webp "Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658](/upload/website/prouct_img/small/GTX637658/GTX637658_T-44837_20221223_IHC-P_M_22122722_497.webp "Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658](/upload/website/prouct_img/small/GTX637658/GTX637658_44900_20221223_WB_22122722_462.webp "Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637658](/upload/website/prouct_img/small/GTX637658/GTX637658_44900_20221230_ICC_IF_22122901_869.webp "Anti-Ataxin 3 antibody [HL1909] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658](/upload/website/prouct_img/small/GTX637658/GTX637658_T-44837_20221118_WB_M_R_22112219_124.webp "Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658](/upload/website/prouct_img/normal/GTX637658/GTX637658_44900_20230210_WB_shRNA_watermark_23021401_927.webp "Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658](/upload/website/prouct_img/normal/GTX637658/GTX637658_T-44837_20221223_IHC-P_22122722_218.webp "Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658](/upload/website/prouct_img/normal/GTX637658/GTX637658_T-44837_20221223_IHC-P_R_22122722_521.webp "Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658](/upload/website/prouct_img/normal/GTX637658/GTX637658_T-44837_20221223_IHC-P_M_22122722_497.webp "Anti-Ataxin 3 antibody [HL1909] used in IHC (Paraffin sections) (IHC-P). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658](/upload/website/prouct_img/normal/GTX637658/GTX637658_44900_20221223_WB_22122722_462.webp "Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637658](/upload/website/prouct_img/normal/GTX637658/GTX637658_44900_20221230_ICC_IF_22122901_869.webp "Anti-Ataxin 3 antibody [HL1909] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637658")
![Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658](/upload/website/prouct_img/normal/GTX637658/GTX637658_T-44837_20221118_WB_M_R_22112219_124.webp "Anti-Ataxin 3 antibody [HL1909] used in Western Blot (WB). GTX637658")
