Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Ataxin 3 antibody [HL1909] detects Ataxin 3 protein by immunofluorescent analysis.
Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.
Blue: Fluoroshield with DAPI (GTX30920).
Scale bar= 10μm.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded mouse stomach.
Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Ataxin 3 antibody [HL1909] detects Ataxin 3 protein at nucleus by immunohistochemical analysis.
Sample: Paraffin-embedded human lung cancer.
Ataxin 3 stained by Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Various tissue extracts (50 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Ataxin 3 antibody [HL1909] (GTX637658) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.