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B-Raf (V600E Mutant Specific)抗体 [RM8]

Orthogonal
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in Western Blot (WB). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595
Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

目录号 GTX33595

文献引用 3

宿主

 Rabbit

克隆

 Monoclonal

克隆编号

 RM8

同种型

 IgG

实验应用

 WB, ICC/IF, IHC-P, ELISA

种属反应

 Human
产品包装
100 μg (¥9200)
查看所有 B-Raf 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 0.5 - 2 μg/mL
ICC/IF 0.5 - 5 μg/mL
IHC-P 0.5 - 5 μg/mL
ELISA 0.5 - 2 μg/mL
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

84 kDa. ( Note )

产品说明

This antibody reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 50% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

Batch dependent (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

A peptide corresponding to BRAF V600E mutant

纯化方式

Protein A purified
From tissue culture supernatant

偶联

Unconjugated

RRID

AB_2887722

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

BRAF,BRAF1,BRaf protooncogene, serine/threonine kinase,Braf,NS7,RAFB1,B-Raf,B-Raf proto-oncogene, serine/threonine kinase

细胞定位

Nucleus,Cytoplasm,Cell membrane

功能与背景

This gene encodes a protein belonging to the RAF family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERK signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene, most commonly the V600E mutation, are the most frequently identified cancer-causing mutations in melanoma, and have been identified in various other cancers as well, including non-Hodgkin lymphoma, colorectal cancer, thyroid carcinoma, non-small cell lung carcinoma, hairy cell leukemia and adenocarcinoma of lung. Mutations in this gene are also associated with cardiofaciocutaneous, Noonan, and Costello syndromes, which exhibit overlapping phenotypes. A pseudogene of this gene has been identified on the X chromosome. [provided by RefSeq, Aug 2017]

数据库

研究领域

產品图片

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in Western Blot (WB). GTX33595

GTX33595 WB Image

Orthogonal

WB analysis of cells expressing endogenous mutant (V600E) BRAF or wild type (WT) protein using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of melanoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of formalin fixed and paraffin embedded sections of SW40 cells (B-Raf Wide type) using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of thyroid Carcinoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of formalin fixed and paraffin embedded sections of WiDr cells (B-Raf V600E mutant) using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

Anti-B-Raf (V600E Mutant Specific) antibody [RM8] used in IHC (Paraffin sections) (IHC-P). GTX33595

GTX33595 IHC-P Image

IHC-P analysis of thyroid Carcinoma tissue using GTX33595 B-Raf (V600E Mutant Specific) antibody [RM8].

文献引用

Application Reference

CP Chen et al. Heliyon 2024; 10 (7) : 29055 Synergistic effects of the combination of trametinib and alpelisib in anaplastic thyroid cancer with BRAF and PI3KCA co-mutations
实验应用 : WB
Makboul R et al. Nucl Med Commun 2020; 41 (5) : 416-425 Do BRAFV600E mutation and sodium-iodide symporter expression affect the response to radioactive iodine therapy in patients with papillary thyroid carcinoma?
实验应用 : IHC-P
种属反应 : Human
Hansen CH et al. Proc Natl Acad Sci U S A 2017; (Epub) Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection.
实验应用 : ELISA
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产品包装 报价 (¥)
¥ 9200
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