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B-Raf抗体 [N2C1], Internal

Overexpression
Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913
Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

目录号 GTX100913

文献引用 5

宿主

 Rabbit

克隆

 Polyclonal

同种型

 IgG

实验应用

 WB, IHC-P, IP

种属反应

 Human, Mouse, Rat
产品包装
100 μl (¥4000),
25 μl (¥1700)
查看所有 B-Raf 的产品

产品应用

应用说明

*最佳稀释倍数与浓度应由研究人员确认
实验应用 稀释倍数
WB 1:500-1:10000
IHC-P 1:100-1:1000
IP 1:100-1:500
WB: 免疫印迹
ICC/IF: 细胞染色
IHC-P: 石蜡切片
IHC-Fr: 冰冻切片

预期分子量

84 kDa. ( Note )

阳性对照

PC-12 , human BRAF-transfected 293T cells

产品属性

形式

Liquid

存储溶液

PBS, 1% BSA, 20% Glycerol

存放说明

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

浓度

0.21 mg/ml (Please refer to the vial label for the specific concentration.)

免疫原种属

Human

免疫原

Recombinant protein encompassing a sequence within the center region of human B-Raf. The exact sequence is proprietary.

纯化方式

Purified by antigen-affinity chromatography.

偶联

Unconjugated

RRID

AB_2036363

注意事项

仅供实验室使用。不适用于人类或动物的任何临床，治疗或诊断用途。不适合动物或人类食用。

相关讯息

别名

B-Raf proto-oncogene, serine/threonine kinase , B-RAF1 , B-raf , BRAF1 , NS7 , RAFB1

细胞定位

Cytoplasm

功能与背景

This gene encodes a protein belonging to the raf/mil family of serine/threonine protein kinases. This protein plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. Mutations in this gene are associated with cardiofaciocutaneous syndrome, a disease characterized by heart defects, mental retardation and a distinctive facial appearance. Mutations in this gene have also been associated with various cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung. A pseudogene, which is located on chromosome X, has been identified for this gene. [provided by RefSeq]

数据库

研究领域

產品图片

Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913

GTX100913 IHC-P Image

B-Raf antibody [N2C1], Internal detects B-Raf protein at cytosol on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-B-Raf antibody [N2C1], Internal used in Immunoprecipitation (IP). GTX100913

GTX100913 IP Image

B-Raf antibody [N2C1], Internal immunoprecipitates B-Raf protein in IP experiments. IP Sample: HepG2 whole cell lysate/extract A : 30 μg whole cell lysate/extract of B-Raf protein expressing HepG2 cells B : Control with 3 μg of pre-immune rabbit IgG C : Immunoprecipitation of B-Raf by 3 μg of B-Raf antibody [N2C1], Internal (GTX100913) 10% SDS-PAGE The immunoprecipitated B-Raf protein was detected by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

Overexpression

B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis. Non-transfected (-) and B-Raf-transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:5000.

Anti-B-Raf antibody [N2C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX100913

GTX100913 IHC-P Image

B-Raf antibody [N2C1], Internal detects B-Raf protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded human breast carcinoma.
B-Raf stained by B-Raf antibody [N2C1], Internal (GTX100913) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-B-Raf antibody [N2C1], Internal used in Western Blot (WB). GTX100913

GTX100913 WB Image

B-Raf antibody [N2C1], Internal detects B-Raf protein by western blot analysis.
A. 30 μg PC-12 whole cell extract
7.5 % SDS-PAGE
B-Raf antibody [N2C1], Internal (GTX100913) dilution: 1:5000

文献引用

Application Reference

CP Chen et al. Heliyon 2024; 10 (7) : 29055 Synergistic effects of the combination of trametinib and alpelisib in anaplastic thyroid cancer with BRAF and PI3KCA co-mutations
实验应用 : WB
Tsuji S et al. Oncol Lett 2021; 21 (2) : 113 Perphenazine exerts antitumor effects on HUT78 cells through Akt dephosphorylation by protein phosphatase 2A.
实验应用 : WB
种属反应 : Human
Tateishi K et al. Acta Neuropathol Commun 2020; 8 (1) : 139 BRAF V600E mutation mediates FDG-methionine uptake mismatch in polymorphous low-grade neuroepithelial tumor of the young.
实验应用 : WB
种属反应 : Human
Sase H et al. Mol Cancer Ther 2018; (Epub) Acquired JHDM1D-BRAF fusion confers resistance to FGFR inhibition in FGFR2- amplified gastric cancer.
实验应用 : WB
种属反应 : Human
Rubin B et al. Cancer Invest 2015; (Epub) Mitogen-Activated Protein Kinase Pathway: Genetic Analysis of 95 Adrenocortical Tumors.
实验应用 : WB
种属反应 : Human
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
产品包装 报价 (¥)
¥ 4000
¥ 1700
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